Importance of Human Faecal Biobanking: From Collection to Storage
Izabela Lewandowska
Pure Clinical Lab Network Ltd. Co.Słupsk, Poland
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, y Jolanta Krzysztoń-Russjan
Department of Medical Biology, National Institute of CardiologyWarsaw, Poland
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29 ene 2025
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Publicado en línea: 29 ene 2025
Páginas: 181 - 189
Recibido: 01 ago 2024
Aceptado: 01 oct 2024
DOI: https://doi.org/10.2478/am-2024-0015
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© 2024 Izabela Lewandowska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Summary of selected storage conditions and preserving faecal samples for a microbiome study_
| Stabilization compounds/solutions | Storage temperature | Storage period | Influence on stability of microbiota | Source |
|---|---|---|---|---|
| Stratec DNA stabilizer | Room temperature | 7 days | Stable microbiota profiles, minimal changes in diversity. | ( |
| OMNIgene-GUT DNA stabilizer | Room temperature | 7 days | Microbiota minimal changes, high DNA stability. | ( |
| DESS (DMSO-EDTA-salt) | Room temperature | 1–3 weeks | Microbiome stability without significant changes in diversity (using targeted extraction methods). | ( |
| Homemade preservation buffer (EDTA, DSD |
Room temperature | 4 weeks | Stability in room temperature (or in high temperatures up to 5 days), ideal when freezing isn’t available. | ( |
| OMNIgene-GUT or NBgene-GUT | Room temperature | 65 days | The best method for room temperature storage is stable microbiome profiles comparable to frozen samples | ( |
| 95% Ethanol, OMNIgene-GUT | Room temperature | 8 weeks | Both methods effectively maintain microbiome stability | ( |
| Ethanol (100%) | Room temperature | 8 weeks | Stable microbiome, alternative to freezing, suitable for long-term storage (study on spider monkey’s stool) | ( |
| No stabilizer | –20°C | 24 h | Minimal changes in taxonomic diversity, good DNA quality. | ( |
| No stabilizer | –20°C (after 48h at 4°C) | 48–96 h | Minimal changes in microbiome and SCFA composition, indicating that 24h refrigeration preserves sample integrity. | ( |
| No stabilizer | –80°C | 48 h | Stable microbiota diversity, high DNA yield, minimal changes in bacterial composition | ( |
| eNAT® medium | –80°C | 30 days at room temperature, then freezing | Stability of microbiome maintained after 30 days at room temperature, no significant changes in diversity and composition | ( |
| 10% Glycerol | –80°C/liquid nitrogen | 12 months | Microbiome stability maintained for 12 months, better diversity than ethanol-preserved samples at room temperature | ( |