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Epigenetic toxicity and cytotoxicity of perfluorooctanoic acid and its effects on gene expression in embryonic mouse hypothalamus cells

Hun Kim
Hun Kim
, 
Min-Wook Hong
Min-Wook Hong
, 
Yun-ho Bae
Yun-ho Bae
 y 
Sung-Jin Lee
Sung-Jin Lee
   | 28 sept 2021
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Article Category: Original article
Publicado en línea: 28 sept 2021
Páginas: 182 - 190
Recibido: 01 may 2021
Aceptado: 01 sept 2021
DOI: https://doi.org/10.2478/aiht-2021-72-3555
© 2021 Hun Kim, Min-Wook Hong, Yun-ho Bae, and Sung-Jin Lee, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Cell viability of mHypoE-N46 cells following PFOA treatment for (A) 24 h and (B) 48 h (MTT assay). Data are shown as mean ± standard error of triplicate measurements. ** p<0.01 and *** p<0.001 compared to control
Cell viability of mHypoE-N46 cells following PFOA treatment for (A) 24 h and (B) 48 h (MTT assay). Data are shown as mean ± standard error of triplicate measurements. ** p<0.01 and *** p<0.001 compared to control

Figure 2

Expression of (A) apoptosis-related genes, (B) cell cycle-related genes, and (C) cell proliferation-related genes after 24 h of exposure to PFOA (qPCR). Data are shown as mean ± standard error of three independent measurements. * p<0.05, ** p<0.01, and *** p<0.001 compared to control
Expression of (A) apoptosis-related genes, (B) cell cycle-related genes, and (C) cell proliferation-related genes after 24 h of exposure to PFOA (qPCR). Data are shown as mean ± standard error of three independent measurements. * p<0.05, ** p<0.01, and *** p<0.001 compared to control

Figure 3

Expression of (A) ligand (Ngf) and receptor (Ntrk1) genes of the NGF-NTRK1 neurotrophin system and (B) ligand (Bdnf) and receptor (Ntrk2) genes of the BDNF-NTRK2 neurotrophin system after 24 h of exposure to PFOA (PCR). Data are shown as mean ± standard error of three independent measurements. * p<0.05, ** p<0.01, and *** p<0.001 compared to control
Expression of (A) ligand (Ngf) and receptor (Ntrk1) genes of the NGF-NTRK1 neurotrophin system and (B) ligand (Bdnf) and receptor (Ntrk2) genes of the BDNF-NTRK2 neurotrophin system after 24 h of exposure to PFOA (PCR). Data are shown as mean ± standard error of three independent measurements. * p<0.05, ** p<0.01, and *** p<0.001 compared to control

Figure 4

Epigenetic toxicity of PFOA: (A) relative global methylation levels; (B) relative expression of ten-eleven translocation methylcytosine dioxygenase-encoding Tet1, Tet2, and Tet3; (C) relative expression of DNA methyltransferase-encoding Dnmt1, Dnmt3a, and Dnmt3b, and (D) relative expression of methyl-CpG binding protein 2 genes. Data are shown as mean ± standard error of three independent measurements. * p<0.05, ** p<0.01, and *** p<0.001 compared to control
Epigenetic toxicity of PFOA: (A) relative global methylation levels; (B) relative expression of ten-eleven translocation methylcytosine dioxygenase-encoding Tet1, Tet2, and Tet3; (C) relative expression of DNA methyltransferase-encoding Dnmt1, Dnmt3a, and Dnmt3b, and (D) relative expression of methyl-CpG binding protein 2 genes. Data are shown as mean ± standard error of three independent measurements. * p<0.05, ** p<0.01, and *** p<0.001 compared to control

List of primer sequences for quantitative real-time polymerase chain reaction

Gene Primer sequence (5′→3′) Amplicon size
Bax Forward: TGCAGAGGATGATTGCTGAC 173 bp
Reverse: GATCAGCTCGGGCACTTTAG
Casp3 Forward: CTGCCGGAGTCTGACTGGAA 97 bp
Reverse: ATCAGTCCCACTGTCTGTCTCAATG
Trp73 (p73) Forward: AGCCTTTGGTTGACTCCTATCG 119 bp
Reverse: ACCACCGTGTACCTTGTTCA
Ccna2 Forward: ACCTGCCTTCACTCATTGCT 139 bp
Reverse: TGGTGAAGGTCCACAAGACA
Ccnb1 Forward: CAGTTGTGTGCCCAAGAAGA 216 bp
Reverse: CTACGGAGGAAGTGCAGAGG
Ccne1 Forward: CACCACTGAGTGCTCCAGAA 230 bp
Reverse: CTGTTGGCTGACAGTGGAGA
Cdkn1a Forward: GGGATGGCAGTTAGGACTCA 244 bp
Reverse: GTGGGGCAAGTGCCTAGATA
Cdk4 Forward: CAATGTTGTACGGCTGATGG 178 bp
Reverse: CAGGCCGCTTAGAAACTGAC
Rps6 Forward: GTCCGCCAGTATGTTGTCAG 103 bp
Reverse: GTTGCAGGACACGAGGAGTA
Kitl Forward: TCATGGTGCACCGTATCCTA 170 bp
Reverse: CCTTGGCATGTTCTTCCACT
Bdnf Forward: GGTATCCAAAGGCCAACTGA 183 bp
Reverse: CTTATGAATCGCCAGCCAAT
Ntrk2 (TrkB) Forward: CGCCCTGTGAGCTGAACTCTG 171 bp
Reverse: CTGCTTCTCAGCTGCCTGACC
Ntrk1 (TrkA) Forward: GAGGTCTCTGTCCAAGTCAGCG 131 bp
Reverse: TTGAACAACCAGCGCAGAGA
Ngf Forward: CAGACCCGCAACATCACTGTA 131 bp
Reverse: CCATGGGCCTGGAAGTCTAG
Tet1 Forward: CGAAAGAACAGCCACCAGAT 219 bp
Reverse: TTGCTCTTCTTCCCCATGAC
Tet2 Forward: GTTGCAAGAAGAAAGCGGAG 229 bp
Reverse: CTCTGCCCTTGCTGAAGGT
Tet3 Forward: TCCGGATTGAGAAGGTCATC 176 bp
Reverse: CCAGGCCAGGATCAAGATAA
Dnmt1 Forward: CCTAGTTCCGTGGCTACGAGGAGAA 136 bp
Reverse: TCTCTCTCCTCTGCAGCCGACTCA
Dnmt3a Forward: CACAGGGCCCGTTACTTCTG 76 bp
Reverse: TCCAGCTTATCATTCACAGTGGAT
Dnmt3b Forward: TTCAGTGACCAGTCCTCAGACACGAA 144 bp
Reverse: TCAGAAGGCTGGAGACCTCCCTCTT
Mecp2_e1 Forward: AGGAGAGACTGGAGGAAAAGT 70 bp
Reverse: CTTAAACTTCAGTGGCTTGTCT
Mecp2_e2 Forward: CTCCATAAAAATACAGACTCACCAGT 183 bp
Reverse: CTTAAACTTCAGTGGCTTGTCT
β-actin Forward: ATGGTGGGAATGGGTCAGAAG 157 bp
Reverse: CACGCAGCTCATTGTAGAAGG
eISSN:
1848-6312
Idiomas:
Inglés, Slovenian
Calendario de la edición:
4 veces al año
Temas de la revista:
Medicine, Basic Medical Science, other
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