<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Sedative phytomedications continue to play an important role in the management of a considerable amount of anxiety symptoms because of the various side effects of synthetic sedatives and tranquilizers. However, developing new herbal drugs needs their appropriate quality control according to the relevant requirements. The aim of the study was to determine the sedative properties of the tinctures obtained from the American basil (<italic>Ocimum americanum</italic> L., <italic>Lamiaceae</italic> Martinov family) herb and to develop the standardization parameters for the promising herbal medicinal product. The open field test was used to evaluate the sedative effect of the prepared tinctures: (1) with the added of <italic>O. americanum</italic> essential oil (OATEs) and (2) without adding <italic>O. americanum</italic> essential oil (OAT). The standardization parameters for the OATEs were developed using validated High-Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC) methods. The HPTLC analysis was used for the chromatographic fingerprints of polyphenols and for identifying linalool in the OATEs. The HPLC analysis found the significant content of rosmarinic acid (RA) (0.26%) in the OATEs. In conclusion, the developed OATEs can be considered as the new herbal medicinal product with significant sedative properties.</p>
</abstract>

Sedative phytomedications continue to play an important role in the management of a considerable amount of anxiety symptoms because of the various side effects of synthetic sedatives and tranquilizers. However, developing new herbal drugs needs their appropriate quality control according to the relevant requirements. The aim of the study was to determine the sedative properties of the tinctures obtained from the American basil (Ocimum americanum L., Lamiaceae Martinov family) herb and to develop the standardization parameters for the promising herbal medicinal product. The open field test was used to evaluate the sedative effect of the prepared tinctures: (1) with the added of O. americanum essential oil (OATEs) and (2) without adding O. americanum essential oil (OAT). The standardization parameters for the OATEs were developed using validated High-Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC) methods. The HPTLC analysis was used for the chromatographic fingerprints of polyphenols and for identifying linalool in the OATEs. The HPLC analysis found the significant content of rosmarinic acid (RA) (0.26%) in the OATEs. In conclusion, the developed OATEs can be considered as the new herbal medicinal product with significant sedative properties.

Sedative Effect and Standardization Parameters of Herbal Medicinal Product Obtained from the <italic>Ocimum americanum</italic> L. Herb

I. Horbachevsky Ternopil National Medical University

European Pharmaceutical Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Sedative Effect and Standardization Parameters of Herbal Medicinal Product Obtained from the  L. Herb

Sedative phytomedications continue to play an important role in the management of a considerable amount of anxiety symptoms because of the various side effects...

Sedative Effect and Standardization Parameters of Herbal Medicinal Product Obtained from the Ocimum americanum L. Herb

Article Category: Original Paper

Publicado en línea: 14 abr 2021

Páginas: 1 - 9

Recibido: 09 sept 2020

Aceptado: 01 feb 2021

DOI: https://doi.org/10.2478/afpuc-2020-0015

Palabras clave
American basil, herb, tincture, sedative activity, polyphenols, linalool, chromatographic analysis

© 2021 Shanaida M. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Sedative Effect and Standardization Parameters of Herbal Medicinal Product Obtained from the Ocimum americanum L. Herb

Article Category: Original Paper

Publicado en línea: 14 abr 2021

Páginas: 1 - 9

Recibido: 09 sept 2020

Aceptado: 01 feb 2021

DOI: https://doi.org/10.2478/afpuc-2020-0015

Palabras claveAmerican basil, herb, tincture, sedative activity, polyphenols, linalool, chromatographic analysis

© 2021 Shanaida M. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
American basil, herb, tincture, sedative activity, polyphenols, linalool, chromatographic analysis