<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Aim</h3><p>evaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (<italic>Beta vulgaris</italic> (L.) subsp. <italic>vulgaris</italic>) on phenylhydrazine-induced rats.</p></sec>
<sec><h3>Methods</h3><p>Male rats were divided into five groups: normal control group, negative control group, dichloromethane extract group, hydroethanolic extract group, and alkaloids-free hydroethanolic extract group. All groups were induced with phenylhydrazine (30 mg.Kg<sup>−1</sup> BW) for three days, except for the normal control group. After induction, each treatment group received each extract (200 mg.Kg<sup>−1</sup> BW) for 21 days. The haematology parameters (haemoglobin levels, the number of erythrocytes, and haematocrit levels) were measured using Haematology Analyzer, and the antioxidant activity was measured through MDA level parameters in rats. Data were analysed using one-way ANOVA and then continued with the Tukey test.</p></sec>
<sec><h3>Results</h3><p>The results showed that the hydroethanolic extract of beetroot increased the percentage of erythrocytes (33.5%), haemoglobin (25%), and haematocrit (24.4%) to the negative control group, which was comparable to the normal control group (p > 0.05). In addition, the best antioxidant activity was shown in the hydroethanolic extract of beetroot, which is comparable to the normal group (p > 0.05).</p></sec>
<sec><h3>Conclusion</h3><p>The beetroot hydroethanolic crude extract could be potentially produced in a natural pharmaceutical product as a beneficial resource within anti-anaemia and antioxidant activities.</p></sec>
</abstract>

Aimevaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (Beta vulgaris (L.) subsp. vulgaris) on phenylhydrazine-induced rats.
MethodsMale rats were divided into five groups: normal control group, negative control group, dichloromethane extract group, hydroethanolic extract group, and alkaloids-free hydroethanolic extract group. All groups were induced with phenylhydrazine (30 mg.Kg−1 BW) for three days, except for the normal control group. After induction, each treatment group received each extract (200 mg.Kg−1 BW) for 21 days. The haematology parameters (haemoglobin levels, the number of erythrocytes, and haematocrit levels) were measured using Haematology Analyzer, and the antioxidant activity was measured through MDA level parameters in rats. Data were analysed using one-way ANOVA and then continued with the Tukey test.
ResultsThe results showed that the hydroethanolic extract of beetroot increased the percentage of erythrocytes (33.5%), haemoglobin (25%), and haematocrit (24.4%) to the negative control group, which was comparable to the normal control group (p > 0.05). In addition, the best antioxidant activity was shown in the hydroethanolic extract of beetroot, which is comparable to the normal group (p > 0.05).
ConclusionThe beetroot hydroethanolic crude extract could be potentially produced in a natural pharmaceutical product as a beneficial resource within anti-anaemia and antioxidant activities.

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

European Pharmaceutical Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Aimevaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (Beta vulgaris...

Groups	Blood Parameters

	Erythrocytes (×10⁶μL)	Haemoglobin (g.dL⁻¹)	Haematocrit (%)

Normal Control	7.00 ± 0.78^a	16.78 ± 0.37^a	50.55 ± 1.22^a
Negative Control	4.73 ± 0.95^b	12.48 ± 0.48^b	37.82 ± 1.49^b
DEB	6.31 ± 0.52^a	14.94 ± 0.46^c	45.18 ± 1.48^c
HEB	7.12 ± 0.61^a	16.66 ± 0.39^a	50.04 ± 1.22^a
AFHEB	6.96 ± 0.55^a	15.46 ± 0.31^c	46.54 ± 0.86^c
Normal Level Value	5.0–12.0	11.1–18.0	36.0–52.0

Compounds	DEB	HEB	AFHEB
Alkaloids	+	+	−
Flavonoids	+	+	+
Tannin	−	+	+
Phenolic	+	+	+
Saponin	−	+	+
Steroids	−	−	−
Triterpenoids	−	−	−

Type of Extract	Iron (Fe) content (mg.Kg⁻¹)	Flavonoids Total Content (mgQE.g⁻¹)
DEB	6.52	1.66
HEB	20.43	8.35
AFHEB	11.30	6.07

Type of Extract	Characteristics
Type of Extract	Percentage of Yield (%, w/w)	Loss of Drying (%, w/w)	Ash Content (%, w/w)
DEB	1.92	2.48	2.16
HEB	34.52	9.89	9.66
AFHEB	24.55	13.27	19.75

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

Article Category: Original Paper

Publicado en línea: 02 abr 2021

Páginas: 1 - 7

Recibido: 19 jun 2020

Aceptado: 01 feb 2021

DOI: https://doi.org/10.2478/afpuc-2020-0014

Palabras clave
Anti-anaemia, Antioxidant, Beta vulgaris, Beetroot, Phenylhydrazine

© 2021 Ni Putu Ermi Hikmawanti et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

The Anti-anaemia Activity of Beetroot Extracts.

Phytochemical Screening Results of Beetroot Extracts.

Flavonoids and Iron Contents of Beetroot Extracts.

The Result of Beetroot Extraction.

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

Article Category: Original Paper

Publicado en línea: 02 abr 2021

Páginas: 1 - 7

Recibido: 19 jun 2020

Aceptado: 01 feb 2021

DOI: https://doi.org/10.2478/afpuc-2020-0014

Palabras claveAnti-anaemia, Antioxidant, Beta vulgaris, Beetroot, Phenylhydrazine

© 2021 Ni Putu Ermi Hikmawanti et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

The Anti-anaemia Activity of Beetroot Extracts.

Phytochemical Screening Results of Beetroot Extracts.

Flavonoids and Iron Contents of Beetroot Extracts.

The Result of Beetroot Extraction.

Palabras clave
Anti-anaemia, Antioxidant, Beta vulgaris, Beetroot, Phenylhydrazine