<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The effect of ionic strength on DNA condensation by cationic liposomes prepared as a mixture of ethane-1,2-diylbis(dodecyl-dimethylammonium bromide) (C2GS12) and dioleoylphosphatidylcholine (DOPC) was studied using fluorescence spectroscopy. The DNA condensation followed by changes in emission intensity of ethidium bromide shows a strong dependence on the ionic strength of the solution. At physiologically relevant ionic strength (0.15 mol/l NaCl), the amount of DNA condensed between lipid bilayers is approximately 40% lower compared to 0.005 mol/l NaCl. The structure of formed complexes was studied using small angle X-ray diffraction (SAXD). DNA–C2GS12–DOPC complexes form a condensed lamellar phase organisation, which is partially disrupted by the increase of ionic strength. Both the lamellar repeat distance and DNA–DNA distance show dependence on C2GS12/DOPC molar ratio, temperature and also on ionic strength. We found that the method of preparation significantly affects both the quality of organisation and the structural parameters of complexes as discussed in the paper.</p></abstract>

The effect of ionic strength on DNA condensation by cationic liposomes prepared as a mixture of ethane-1,2-diylbis(dodecyl-dimethylammonium bromide) (C2GS12) and dioleoylphosphatidylcholine (DOPC) was studied using fluorescence spectroscopy. The DNA condensation followed by changes in emission intensity of ethidium bromide shows a strong dependence on the ionic strength of the solution. At physiologically relevant ionic strength (0.15 mol/l NaCl), the amount of DNA condensed between lipid bilayers is approximately 40% lower compared to 0.005 mol/l NaCl. The structure of formed complexes was studied using small angle X-ray diffraction (SAXD). DNA–C2GS12–DOPC complexes form a condensed lamellar phase organisation, which is partially disrupted by the increase of ionic strength. Both the lamellar repeat distance and DNA–DNA distance show dependence on C2GS12/DOPC molar ratio, temperature and also on ionic strength. We found that the method of preparation significantly affects both the quality of organisation and the structural parameters of complexes as discussed in the paper.

DNA – DOPC – gemini surfactants complexes: effect of ionic strength

Comenius University in Bratislava, Faculty of Pharmacy, Department of Physical Chemistry of drugs, Slovak republic

Charles University, Faculty of Pharmacy, Department of Inorganic and Organic Chemistry, Hradec Králové, Czech republic

Comenius University in Bratislava, Faculty of Pharmacy, Department of Chemical Theory of Drugs, Slovak republic

European Pharmaceutical Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"DNA \u2013 DOPC \u2013 gemini surfactants complexes: effect of ionic strength"}

The effect of ionic strength on DNA condensation by cationic liposomes prepared as a mixture of ethane-1,2-diylbis(dodecyl-dimethylammonium bromide) (C2GS12)...

DNA – DOPC – gemini surfactants complexes: effect of ionic strength

Publicado en línea: 30 dic 2014

Páginas: 26 - 34

DOI: https://doi.org/10.2478/afpuc-2014-0013

Palabras clave
DNA, Gemini surfactants, dioleoylphosphatidylcholine, Fluorescence spectroscopy, Small angle X-ray diffraction

© Acta Facultatis Pharmaceuticae Universitatis Comenianae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

DNA – DOPC – gemini surfactants complexes: effect of ionic strength

Publicado en línea: 30 dic 2014

Páginas: 26 - 34

DOI: https://doi.org/10.2478/afpuc-2014-0013

Palabras claveDNA, Gemini surfactants, dioleoylphosphatidylcholine, Fluorescence spectroscopy, Small angle X-ray diffraction

© Acta Facultatis Pharmaceuticae Universitatis Comenianae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
DNA, Gemini surfactants, dioleoylphosphatidylcholine, Fluorescence spectroscopy, Small angle X-ray diffraction