<abstract xmlns="http://www.w3.org/1999/xhtml">The micellization process of the local anaesthetic carbisocainium chloride in water was investigated by two spectral methods
using the probe pyrene. First, the absorption spectroscopy in UV/VIS region was based on studying changes in characteristic
absorption spectrum of pyrene in presence of surfactant. The resultant plot of the sum of absorbances for all the major pyrene
peaks as a function of the total surfactant concentration shows, around the critical micellar concentration, a typical sigmoidal
increase. The fluorescence emission spectroscopy in UV/VIS region of spectrum by the probe pyrene, second procedure, was applied
for determination of the cmc from the measurements of the pyrene <italic>I1 /I3</italic> ratio as a function of the surfactant concentration.
The pyrene ratio data were fitted by the Boltzmann-type sigmoid of decreasing character.</abstract>

The micellization process of the local anaesthetic carbisocainium chloride in water was investigated by two spectral methods
using the probe pyrene. First, the absorption spectroscopy in UV/VIS region was based on studying changes in characteristic
absorption spectrum of pyrene in presence of surfactant. The resultant plot of the sum of absorbances for all the major pyrene
peaks as a function of the total surfactant concentration shows, around the critical micellar concentration, a typical sigmoidal
increase. The fluorescence emission spectroscopy in UV/VIS region of spectrum by the probe pyrene, second procedure, was applied
for determination of the cmc from the measurements of the pyrene I1 /I3 ratio as a function of the surfactant concentration.
The pyrene ratio data were fitted by the Boltzmann-type sigmoid of decreasing character.

A Study of Local Anaesthetics. Part 202.
Determination of the Critical Micellar Concentration of Carbisocainium
Chloride in Water Using Spectral Methods and the Probe Pyrene

Comenius University in Bratislava, Faculty of Pharmacy,
Department of Pharmaceutical Chemistry, Odbojárov 10,
832 32 Bratislava, Slovak Republic

Comenius University in Bratislava, Faculty of Pharmacy,
Department of Physical Chemistry of Drugs

European Pharmaceutical Journal

The micellization process of the local anaesthetic carbisocainium chloride in water was investigated by two spectral methods
using the probe pyrene. First, the...

A Study of Local Anaesthetics. Part 202. Determination of the Critical Micellar Concentration of Carbisocainium Chloride in Water Using Spectral Methods and the Probe Pyrene

Publicado en línea: 27 jun 2013

Páginas: 1 - 6

DOI: https://doi.org/10.2478/afpuc-2013-0024

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