The objective of the study was to determine self-perceived fatigue (S-PF) after motor abilities testing (MAT) in adolescent male and female elite tennis players. The research sample comprised of 17 male (mean age of 13.00 ± 1.54 years) and 20 female (mean age of 13.15 ± 1.42 years) adolescent elite tennis players who underwent testing of selected motor abilities in tennis. The level of fatigue in adolescent elite tennis players was measured with the Training Distress Scale (TDS). Both groups of elite tennis players declared the lowest, zero level of S-PF by TDS symptoms as inability to remember things and loose bowels or diarrhoea and significant S-PF during 48 hours after MAT was in both genders felt by lack of energy, feeling of heavy arms and legs, joint stiffness of soreness, and muscle soreness. Boys versus girls experienced significantly higher S-PF in the symptoms as difficulty falling asleep, being unusually tired during the day, joint stiffness of soreness, and ordinary tasks require extra effort. No significant differences were found in overall TDS between males and females adolescent elite tennis players.