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<p>The effects of propylthiouracil (PTU) treatment and selenium-deficient diet on selenium and thyroid status of Wistar male rats were examined in this study. Wistar male rats (n =128) were divided into four groups: (1) control group – selenium-adequate rats fed a diet supplemented with 0.334 mg Na selenite/kg feed and received regular drinking water (Se+PTU-); (2) selenium adequate rats fed a diet supplemented with 0.334 mg Na selenite/kg feed and received a dose of 150 mg/L of PTU in drinking water, (Se+PTU+); (3) selenium-deficient rats fed a diet containing 0.031 mg Na selenite/ kg and received regular drinking water (Se-PTU-); (4) selenium deficient rats fed a diet containing 0.031 mg Na selenite/kg and received 150 mg/L of PTU in drinking water (Se-PTU+). After three and seven weeks of treatment, all Se – animals had significantly lower whole blood Se concentrations and GPx1 and GPx3 activities. PTU induced a significant decrease in T4 and T3 plasma concentrations after three weeks of treatment in both PTU+ groups. Furthermore, after seven weeks, the T3 level was close to its detection limit in Se – animals. A negative correlation was spotted between GPx activity and concentration of T3 after three and seven weeks. It could indicate an inhibitory influence of thyroid hormones on the expression and/or activities of GPx enzymes related to the available Se in conditions of systemic decrease of T4 concentration. This effect was particularly pronounced in Se – animals.</p>
</abstract>

The effects of propylthiouracil (PTU) treatment and selenium-deficient diet on selenium and thyroid status of Wistar male rats were examined in this study. Wistar male rats (n =128) were divided into four groups: (1) control group – selenium-adequate rats fed a diet supplemented with 0.334 mg Na selenite/kg feed and received regular drinking water (Se+PTU-); (2) selenium adequate rats fed a diet supplemented with 0.334 mg Na selenite/kg feed and received a dose of 150 mg/L of PTU in drinking water, (Se+PTU+); (3) selenium-deficient rats fed a diet containing 0.031 mg Na selenite/ kg and received regular drinking water (Se-PTU-); (4) selenium deficient rats fed a diet containing 0.031 mg Na selenite/kg and received 150 mg/L of PTU in drinking water (Se-PTU+). After three and seven weeks of treatment, all Se – animals had significantly lower whole blood Se concentrations and GPx1 and GPx3 activities. PTU induced a significant decrease in T4 and T3 plasma concentrations after three weeks of treatment in both PTU+ groups. Furthermore, after seven weeks, the T3 level was close to its detection limit in Se – animals. A negative correlation was spotted between GPx activity and concentration of T3 after three and seven weeks. It could indicate an inhibitory influence of thyroid hormones on the expression and/or activities of GPx enzymes related to the available Se in conditions of systemic decrease of T4 concentration. This effect was particularly pronounced in Se – animals.

Effects of Combined Hypothyroidism and Selenium Deficiency on Selenoenzyme Glutathione Peroxidase Activity in Rats

Faculty of Veterinary Medicine, Department of Physiology and Biochemistry

Faculty of Veterinary Medicine, Department of Pathology

Faculty of Veterinary Medicine, Department of Pathophysiology

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

The effects of propylthiouracil (PTU) treatment and selenium-deficient diet on selenium and thyroid status of Wistar male rats were examined in this study....

Effects of Combined Hypothyroidism and Selenium Deficiency on Selenoenzyme Glutathione Peroxidase Activity in Rats

Article Category: Research article

Publicado en línea: 24 jun 2024

Páginas: 261 - 272

Recibido: 10 dic 2023

Aceptado: 30 may 2024

DOI: https://doi.org/10.2478/acve-2024-0018

Palabras clave
glutathione peroxidase, propylthiouracil, rats, selenium, thyroid hormones

© 2024 Svetlana Milanović et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effects of Combined Hypothyroidism and Selenium Deficiency on Selenoenzyme Glutathione Peroxidase Activity in Rats

Article Category: Research article

Publicado en línea: 24 jun 2024

Páginas: 261 - 272

Recibido: 10 dic 2023

Aceptado: 30 may 2024

DOI: https://doi.org/10.2478/acve-2024-0018

Palabras claveglutathione peroxidase, propylthiouracil, rats, selenium, thyroid hormones

© 2024 Svetlana Milanović et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Palabras clave
glutathione peroxidase, propylthiouracil, rats, selenium, thyroid hormones