The Electrophoretic Pattern of Serum Proteins in Sheep with Naturally Acquired Gastrointestinal Nematode Infections

The objective of this study was to describe the serum protein pattern in sheep with naturally acquired gastrointestinal parasitosis and to compare the distribution of protein fractions with the results obtained in animals with negative fecal examination results. Fecal and blood samples were taken from twenty-nine sheep positive for nematode eggs and twenty-four animals with negative results of fecal examination. Between the evaluated groups of sheep significant differences were noted in the relative mean values for all protein fractions and for albumin/globulin ratio (p<0.01 and p<0.001). The concentrations of total proteins showed no significant differences between both groups of sheep. The absolute mean values of albumin, α₂-globulins and A/G ratio were significantly lower, the mean concentrations of α₁-, β-, γ₁- and γ₂-globulins significantly higher in the nematode positive group of sheep (p<0.01 and p<0.001). In the nematode positive group the protein electrophoretic pattern showed a double α₂-zone in three sheep and the γ-globulin zones were characterized by a diffuse higher broad and wide peaks. The presented results indicate that the gastrointestinal parasitic infections in sheep alter the distribution of serum proteins, and suggest their usefulness in animals with unchanged serum protein concentrations. The study brings new findings and extends the knowledge about the metabolic responses and consequences of gastrointestinal parasitic infections in sheep, particularly with regard to alterations in protein metabolism.