<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Bacillus cereus,</italic> usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections can also occur. The aim was to detect genes for enterotoxins (<italic>hbl</italic>A, <italic>entFM</italic>) and emetic toxin (<italic>cer</italic>) and to investigate the genetic heterogeneity of <italic>B. cereus</italic> isolates from food, environment and human stool. Identification of <italic>B. cereus</italic> was performed by means of selective medium, classical biochemical test and polymerase chain reaction (PCR). Toxin genes were detected by PCR. Typing was performed by random amplified polymorphic DNA (RAPD). <italic>EntFM</italic> gene was present in all stool and food samples and in 28/30 environmental isolates. <italic>Hbl</italic>A gene was present in 29/30 stool, 23/30 food and 24/30 environmental isolates. <italic>Cer</italic> gene was present in 30/30 stool, 28/30 food and 25/30 environmental isolates. The RAPD results show high heterogeneity among the isolates from each group. In the cumulative dendrogram, representative isolates from all three groups formed two clusters with a difference of 53%. The detection of toxin genes in all <italic>B. cereus</italic> isolates indicated these bacteria as potentially pathogenic and a serious threat for human health. The presence of isolates from all three groups in the same cluster suggests the existence of similar strains in the environment, food and patients, which is in line with the circulation of strains in nature through the food chain.</p></abstract>

Bacillus cereus, usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections can also occur. The aim was to detect genes for enterotoxins (hblA, entFM) and emetic toxin (cer) and to investigate the genetic heterogeneity of B. cereus isolates from food, environment and human stool. Identification of B. cereus was performed by means of selective medium, classical biochemical test and polymerase chain reaction (PCR). Toxin genes were detected by PCR. Typing was performed by random amplified polymorphic DNA (RAPD). EntFM gene was present in all stool and food samples and in 28/30 environmental isolates. HblA gene was present in 29/30 stool, 23/30 food and 24/30 environmental isolates. Cer gene was present in 30/30 stool, 28/30 food and 25/30 environmental isolates. The RAPD results show high heterogeneity among the isolates from each group. In the cumulative dendrogram, representative isolates from all three groups formed two clusters with a difference of 53%. The detection of toxin genes in all B. cereus isolates indicated these bacteria as potentially pathogenic and a serious threat for human health. The presence of isolates from all three groups in the same cluster suggests the existence of similar strains in the environment, food and patients, which is in line with the circulation of strains in nature through the food chain.

Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of <italic>Bacillus cereus</italic> Isolated from Food, Environmental and Clinical Samples in Serbia

Institute of Microbiology, Military Medical Academy

Institute of Hygiene, Military Medical Academy

Department of Microbiology, Institute of Soil Science

Institute of Epidemiology, Military Medical Academy

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of  Isolated from Food, Environmental and Clinical Samples in Serbia

Bacillus cereus, usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections...

Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia

Publicado en línea: 08 jun 2020

Páginas: 182 - 193

Recibido: 14 jun 2019

Aceptado: 16 mar 2020

DOI: https://doi.org/10.2478/acve-2020-0013

Palabras clave
enterotoxin genes, emetic toxin gene, PCR, RAPD typing

© 2020 Savić Dejana et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia

Publicado en línea: 08 jun 2020

Páginas: 182 - 193

Recibido: 14 jun 2019

Aceptado: 16 mar 2020

DOI: https://doi.org/10.2478/acve-2020-0013

Palabras claveenterotoxin genes, emetic toxin gene, PCR, RAPD typing

© 2020 Savić Dejana et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
enterotoxin genes, emetic toxin gene, PCR, RAPD typing