<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p=0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (−0.54°C) had significantly higher (p=0.004) average FPD values than those with AB genotype (−0.58°C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.</p></abstract>

Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p=0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (−0.54°C) had significantly higher (p=0.004) average FPD values than those with AB genotype (−0.58°C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.

Insight in Leptin Gene Polymorphism and Impact on Milk Traits in Autochtonous Busha Cattle

Faculty of Veterinary medicine, Department of Reproduction, Fertility and Artificial Insemination

Faculty of Veterinary medicine, Department of Physiology and Biochemistry

Veterinary Faculty, Department of Hemistry, Biochemistry and Physiology

Faculty of Agriculture, Department of Nutrition, Physiology and Anatomy of domestic and farm animals

Faculty of Veterinary medicine, Department of Biology

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect...

Insight in Leptin Gene Polymorphism and Impact on Milk Traits in Autochtonous Busha Cattle

Publicado en línea: 22 jun 2019

Páginas: 153 - 163

Recibido: 21 mar 2019

Aceptado: 13 may 2019

DOI: https://doi.org/10.2478/acve-2019-0012

Palabras clave
Busha cattle, leptin polymorphsm, milk traits, genetic markers

© 2019 Maletić Milan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Insight in Leptin Gene Polymorphism and Impact on Milk Traits in Autochtonous Busha Cattle

Publicado en línea: 22 jun 2019

Páginas: 153 - 163

Recibido: 21 mar 2019

Aceptado: 13 may 2019

DOI: https://doi.org/10.2478/acve-2019-0012

Palabras claveBusha cattle, leptin polymorphsm, milk traits, genetic markers

© 2019 Maletić Milan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
Busha cattle, leptin polymorphsm, milk traits, genetic markers