<abstract xmlns="http://www.w3.org/1999/xhtml">

The novelty of this work is the simultaneous analysis of sulbactam (SUL), ampicillin (AMP), and paracetamol (PARA) in human urine samples, using the environmentally benign RP-HPLC method. A C18 column was used in chromatographic separation using potassium dihydrogen phosphate (10 mmol L–1, pH 5)/ethanol (90 %, <italic>V</italic>/<italic>V</italic>) as the mobile phase; flow rate was 1.00 mL min–1. UV detection at 220 nm was used for quantification. The proposed method showed good linearity in the concentration ranges of 2.20–250.00 μg mL–1 for SUL, 2.50–250.00 μg mL–1 for PARA, and 14.50–250.00 μg mL–1 for AMP. Direct injection of urine samples with no prior extraction was performed. This method was found successful in moving towards greener studies of drugs’ urinary excretion, by decreasing hazardous solvent consumption and waste. Moreover, the method was applied to investigate the urinary excretion of the drugs and possible interaction between ampicillin and paracetamol.
</abstract>

The novelty of this work is the simultaneous analysis of sulbactam (SUL), ampicillin (AMP), and paracetamol (PARA) in human urine samples, using the environmentally benign RP-HPLC method. A C18 column was used in chromatographic separation using potassium dihydrogen phosphate (10 mmol L–1, pH 5)/ethanol (90 %, V/V) as the mobile phase; flow rate was 1.00 mL min–1. UV detection at 220 nm was used for quantification. The proposed method showed good linearity in the concentration ranges of 2.20–250.00 μg mL–1 for SUL, 2.50–250.00 μg mL–1 for PARA, and 14.50–250.00 μg mL–1 for AMP. Direct injection of urine samples with no prior extraction was performed. This method was found successful in moving towards greener studies of drugs’ urinary excretion, by decreasing hazardous solvent consumption and waste. Moreover, the method was applied to investigate the urinary excretion of the drugs and possible interaction between ampicillin and paracetamol.

A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine

Pharmaceutical Chemistry Department Faculty of Pharmacy

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy

Department of Pharmacology & Toxicology Faculty of Pharmacy

Department of Pharmaceutical Chemistry Faculty of Pharmacy

Pharmaceutical Chemistry Department Faculty of Pharma

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The novelty of this work is the simultaneous analysis of sulbactam (SUL), ampicillin (AMP), and paracetamol (PARA) in human urine samples, using the...

A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine

Article Category: Original research paper

Publicado en línea: 30 dic 2021

Páginas: 259 - 274

Aceptado: 04 may 2021

DOI: https://doi.org/10.2478/acph-2022-0015

Palabras clave
ampicillin, paracetamol, sulbactam, HPLC, green analytical chemistry, drug-drug interaction, urinary excretion

© 2022 Basma M. Selim., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

A green approach to the analysis of co-administered ampicillin/sulbactam and paracetamol in human urine

Article Category: Original research paper

Publicado en línea: 30 dic 2021

Páginas: 259 - 274

Aceptado: 04 may 2021

DOI: https://doi.org/10.2478/acph-2022-0015

Palabras claveampicillin, paracetamol, sulbactam, HPLC, green analytical chemistry, drug-drug interaction, urinary excretion

© 2022 Basma M. Selim., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
ampicillin, paracetamol, sulbactam, HPLC, green analytical chemistry, drug-drug interaction, urinary excretion