<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree <italic>Nicotiana glauca</italic> Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (<italic>m</italic>/<italic>m</italic>) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g<sup>−1</sup> dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of <italic>N. glauca</italic>. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (<italic>e.g</italic>., <italic>β</italic>-bisabolol) and carboxylic acids and esters (<italic>e.g</italic>., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.</p>
</abstract>

The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g−1 dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of N. glauca. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (e.g., β-bisabolol) and carboxylic acids and esters (e.g., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.

UPLC-HRESI-MS and GC-MS analysis of the leaves of <italic>Nicotiana glauca</italic>

Department of Pharmaceutical Technology, Faculty of Pharmacy

Jordan University of Science and Technology

Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy

Department of Plant Production, Faculty of Agriculture

Department of Pharmaceutical Sciences, Faculty of Pharmacy

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

UPLC-HRESI-MS and GC-MS analysis of the leaves of 

The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae)...

UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca

Publicado en línea: 30 ago 2021

Páginas: 97 - 108

Aceptado: 27 oct 2020

DOI: https://doi.org/10.2478/acph-2022-0001

Palabras clave
tobacco tree, UPLC-HRESIMS, GC-MS, anabasine, nicotine, volatile oil, methanolic extract

© 2022 Rafeef K. Massadeh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca

Publicado en línea: 30 ago 2021

Páginas: 97 - 108

Aceptado: 27 oct 2020

DOI: https://doi.org/10.2478/acph-2022-0001

Palabras clavetobacco tree, UPLC-HRESIMS, GC-MS, anabasine, nicotine, volatile oil, methanolic extract

© 2022 Rafeef K. Massadeh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
tobacco tree, UPLC-HRESIMS, GC-MS, anabasine, nicotine, volatile oil, methanolic extract