<abstract xmlns="http://www.w3.org/1999/xhtml">This study is aimed to investigate the <italic>in vitro</italic> anti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, <italic>Nectandra megapotamica</italic>, <italic>Brunfelsia uniflora</italic>, <italic>Myrcianthes pungens</italic>, <italic>Anona muricata</italic>, <italic>Hymenaea stigonocarpa</italic> and <italic>Piper corcovandesis</italic>. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms of <italic>Leishmania amazonensis</italic> and <italic>Leishmania braziliensis</italic> and the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results for <italic>L. amazonensis</italic> and <italic>L. braziliensis</italic> were shown by the ethanolic extract of the fruits of <italic>Hymenaea stigonocarpa</italic> and dichloromethane extract of the roots of <italic>Piper corcovadensis</italic>, with <italic>IC</italic>50 of 160 and 150 μg mL−1, resp. Ethanolic extracts of <italic>A. muricata</italic> (leaf), <italic>B. uniflora</italic> (flower and leaf), <italic>M. pungens</italic> (fruit and leaf), <italic>N. megapotamica</italic> (leaf), and aqueous extract of <italic>H. stigonocarpa</italic> (fruit) showed <italic>IC</italic>50 > 170 μg mL−1 for <italic>L. amazonensis</italic> and > 200 μg mL−1 for <italic>L. braziliensis</italic>. The extracts exhibited low cytotoxicity towards J774.A1 macrophages with <italic>CC</italic>50 > 1000 μg mL−1 and hemolytic activity from 0 to 46.1 %.</abstract>

This study is aimed to investigate the in vitro anti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, Nectandra megapotamica, Brunfelsia uniflora, Myrcianthes pungens, Anona muricata, Hymenaea stigonocarpa and Piper corcovandesis. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms of Leishmania amazonensis and Leishmania braziliensis and the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results for L. amazonensis and L. braziliensis were shown by the ethanolic extract of the fruits of Hymenaea stigonocarpa and dichloromethane extract of the roots of Piper corcovadensis, with IC50 of 160 and 150 μg mL−1, resp. Ethanolic extracts of A. muricata (leaf), B. uniflora (flower and leaf), M. pungens (fruit and leaf), N. megapotamica (leaf), and aqueous extract of H. stigonocarpa (fruit) showed IC50 > 170 μg mL−1 for L. amazonensis and > 200 μg mL−1 for L. braziliensis. The extracts exhibited low cytotoxicity towards J774.A1 macrophages with CC50 > 1000 μg mL−1 and hemolytic activity from 0 to 46.1 %.

Screening of six medicinal plant species for antileishmanial activity

Posgraduate Program in Health Sciences Maringá State University

Posgraduate Program in Pharmaceutical Sciences, Maringá State University

Postgraduate Program in Chemistry, Maringá State University

Posgraduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense UNIPAR

Posgraduate Program in Animal Science of the University of Paranaense

Department of Clinical Analysis and Biomedicine

Maringá State University Maringá – Paraná State

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Screening of six medicinal plant species for antileishmanial activity"}

This study is aimed to investigate the in vitro anti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or...

Screening of six medicinal plant species for antileishmanial activity

Publicado en línea: 31 dic 2020

Páginas: 399 - 414

Aceptado: 23 jul 2020

DOI: https://doi.org/10.2478/acph-2021-0023

Palabras clave
Piper corcovadensis, Hymenaea stigonocarpa, antileishmanial, Leishmania amazonensis, Leishmania braziliensis, cytotoxicity, hemolysis

© 2021 Fabiana Borges Padilha Ferreira et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Screening of six medicinal plant species for antileishmanial activity

Publicado en línea: 31 dic 2020

Páginas: 399 - 414

Aceptado: 23 jul 2020

DOI: https://doi.org/10.2478/acph-2021-0023

Palabras clavePiper corcovadensis, Hymenaea stigonocarpa, antileishmanial, Leishmania amazonensis, Leishmania braziliensis, cytotoxicity, hemolysis

© 2021 Fabiana Borges Padilha Ferreira et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
Piper corcovadensis, Hymenaea stigonocarpa, antileishmanial, Leishmania amazonensis, Leishmania braziliensis, cytotoxicity, hemolysis