<abstract xmlns="http://www.w3.org/1999/xhtml"><p>New methods for assaying trimetazidine dihydrochloride on the basis of thin layer chromatography and spectrophotometry are proposed and compared in the paper. In HPTLC/UV-densitometry, separation is achieved by using a mobile phase composed of ammonia-methanol (30:70, <italic>V</italic>/<italic>V</italic>) on silica gel HPTLC plates F254. Quantification using a non-linear calibration curve is accomplished by densito-metric detection at 230 nm. Derivative spectrophotometric determination of trimetazidine dihydrochloride is carried out from the fourth derivative of the absorbance at 233 nm in peak-zero mode. Statistical comparison led to the conclusion that there is no significant difference between the two studied methods and, moreover, that they demonstrate satisfactory accuracy and precision for routine applications.</p></abstract>

New methods for assaying trimetazidine dihydrochloride on the basis of thin layer chromatography and spectrophotometry are proposed and compared in the paper. In HPTLC/UV-densitometry, separation is achieved by using a mobile phase composed of ammonia-methanol (30:70, V/V) on silica gel HPTLC plates F254. Quantification using a non-linear calibration curve is accomplished by densito-metric detection at 230 nm. Derivative spectrophotometric determination of trimetazidine dihydrochloride is carried out from the fourth derivative of the absorbance at 233 nm in peak-zero mode. Statistical comparison led to the conclusion that there is no significant difference between the two studied methods and, moreover, that they demonstrate satisfactory accuracy and precision for routine applications.

Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations

Department of Toxicology, Faculty of Pharmacy

Collegium Medicum of Nicolaus Copernicus University

Department of Geriatrics, Collegium Medicum in Bydgoszcz

Medical Physics Division, Biophysics Department, Faculty of Pharmacy Collegium Medicum

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

New methods for assaying trimetazidine dihydrochloride on the basis of thin layer chromatography and spectrophotometry are proposed and compared in the paper....

Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations

Publicado en línea: 26 jun 2019

Páginas: 413 - 422

Aceptado: 21 ene 2019

DOI: https://doi.org/10.2478/acph-2019-0028

Palabras clave
trimetazidine dihydrochloride, high performance thin layer chromatography/UV-densitometry, derivative spectrophotometry, pharmaceutical formulations

© 2019 Marcin Gackowski et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations

Publicado en línea: 26 jun 2019

Páginas: 413 - 422

Aceptado: 21 ene 2019

DOI: https://doi.org/10.2478/acph-2019-0028

Palabras clavetrimetazidine dihydrochloride, high performance thin layer chromatography/UV-densitometry, derivative spectrophotometry, pharmaceutical formulations

© 2019 Marcin Gackowski et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
trimetazidine dihydrochloride, high performance thin layer chromatography/UV-densitometry, derivative spectrophotometry, pharmaceutical formulations