<abstract xmlns="http://www.w3.org/1999/xhtml"> A simple RP HPLC method for quantification of betamethasone dipropionate (BDP) in gingival crevicular fluid (GCF) has been developed and validated. GCF represents a valuable matrix for therapeutic monitoring of drugs used in the treatment of periodontal disease. The proposed method involves single step extraction for sample preparation. The calibration curve for BDP was linear over the concentration range of 0.10-2.00 μg mL-1 (R2 = 0.9971). RSD values of intra- and inter-day precision ranged 2.2-4.5 and 1.6-5.7 %, while accuracy values were higher than 96.6 and 97.0 %, respectively. The described method can be successfully applied for determination of betamethasone concentrations in GCF obtained from patients with chronic periodontitis after local treatment with BDP cream 0.5 mg g-1.</abstract>

A simple RP HPLC method for quantification of betamethasone dipropionate (BDP) in gingival crevicular fluid (GCF) has been developed and validated. GCF represents a valuable matrix for therapeutic monitoring of drugs used in the treatment of periodontal disease. The proposed method involves single step extraction for sample preparation. The calibration curve for BDP was linear over the concentration range of 0.10-2.00 μg mL-1 (R2 = 0.9971). RSD values of intra- and inter-day precision ranged 2.2-4.5 and 1.6-5.7 %, while accuracy values were higher than 96.6 and 97.0 %, respectively. The described method can be successfully applied for determination of betamethasone concentrations in GCF obtained from patients with chronic periodontitis after local treatment with BDP cream 0.5 mg g-1.

Development and validation of RP HPLC method for determination of betamethasone dipropionate in gingival crevicular fluid

University »Ss Cyril and Methodius« Faculty of Pharmacy, Skopje Republic of Macedonia

University »Ss Cyril and Methodius« Faculty of Dentistry, Skopje Republic of Macedonia

Acta Pharmaceutica

A simple RP HPLC method for quantification of betamethasone dipropionate (BDP) in gingival crevicular fluid (GCF) has been developed and validated. GCF...

Development and validation of RP HPLC method for determination of betamethasone dipropionate in gingival crevicular fluid

Publicado en línea: 22 oct 2013

Páginas: 419 - 426

DOI: https://doi.org/10.2478/acph-2013-0030

Palabras clave
betamethasone dipropionate, gingival crevicular fluid, HPLC, periodontal disease

This content is open access.

Development and validation of RP HPLC method for determination of betamethasone dipropionate in gingival crevicular fluid

Publicado en línea: 22 oct 2013

Páginas: 419 - 426

DOI: https://doi.org/10.2478/acph-2013-0030

Palabras clavebetamethasone dipropionate, gingival crevicular fluid, HPLC, periodontal disease

This content is open access.

Palabras clave
betamethasone dipropionate, gingival crevicular fluid, HPLC, periodontal disease