<abstract xmlns="http://www.w3.org/1999/xhtml">A reliable, selective and sensitive LC-MS/MS assay has been proposed for the determination of nitrofurantoin in human plasma. The analyte and nitrofurazone were extracted from 100 μL of human plasma via SPE on Strata-X 33 μm extraction cartridges. Chromatography was done on a BDS Hypersil C18 (100 mm × 4.6 mm, 5 μm) column under isocratic conditions. Quantitation was done using the multiple reaction monitoring (MRM) mode for deprotonated precursor to product ion transitions of nitrofurantoin (m/z 237.0 → 151.8) and nitrofurazone (m/z 197.0 → 123.9). The limit of detection and the lowest limit of quantitation of the method were 0.25 ng mL-1 and 5.00 ng mL-1, respectively, with a linear dynamic range of 5.00-1500 ng mL-1 for nitrofurantoin. The intra- -batch and inter-batch precision (RSD, %) was ≤ 5.8 %, while the mean extraction recovery was > 92 %. The method was successfully applied to a bioequivalence study of a 100 mg nitrofurantoin capsule formulation in 36 healthy subjects. </abstract>

A reliable, selective and sensitive LC-MS/MS assay has been proposed for the determination of nitrofurantoin in human plasma. The analyte and nitrofurazone were extracted from 100 μL of human plasma via SPE on Strata-X 33 μm extraction cartridges. Chromatography was done on a BDS Hypersil C18 (100 mm × 4.6 mm, 5 μm) column under isocratic conditions. Quantitation was done using the multiple reaction monitoring (MRM) mode for deprotonated precursor to product ion transitions of nitrofurantoin (m/z 237.0 → 151.8) and nitrofurazone (m/z 197.0 → 123.9). The limit of detection and the lowest limit of quantitation of the method were 0.25 ng mL-1 and 5.00 ng mL-1, respectively, with a linear dynamic range of 5.00-1500 ng mL-1 for nitrofurantoin. The intra- -batch and inter-batch precision (RSD, %) was ≤ 5.8 %, while the mean extraction recovery was > 92 %. The method was successfully applied to a bioequivalence study of a 100 mg nitrofurantoin capsule formulation in 36 healthy subjects.

Quantitation of nitrofurantoin in human plasma by liquid chromatography tandem mass spectrometry

Chemistry Department Pramukh Swami Science and H.D. Patel Arts College Sarva Vidyalaya Campus Kadi-382715, Gujarat, India

Bio-Analytical Laboratory Cliantha Research Ltd., Bodakdev Ahmedabad-380054, Gujarat, India

Department of Chemistry School of Sciences, Gujarat University Navrangpura, Ahmedabad-380009 Gujarat, India

Department of Chemistry St. Xavier’s College, Navrangpura Ahmedabad-380009, Gujarat, India

Acta Pharmaceutica

A reliable, selective and sensitive LC-MS/MS assay has been proposed for the determination of nitrofurantoin in human plasma. The analyte and nitrofurazone...

Quantitation of nitrofurantoin in human plasma by liquid chromatography tandem mass spectrometry

Publicado en línea: 10 jul 2013

Páginas: 141 - 158

DOI: https://doi.org/10.2478/acph-2013-0012

Palabras clave
nitrofurantoin, LC-MS/MS, solid phase extraction, human plasma, bioequivalence, incurred sample reanalysis

This content is open access.

Quantitation of nitrofurantoin in human plasma by liquid chromatography tandem mass spectrometry

Publicado en línea: 10 jul 2013

Páginas: 141 - 158

DOI: https://doi.org/10.2478/acph-2013-0012

Palabras clavenitrofurantoin, LC-MS/MS, solid phase extraction, human plasma, bioequivalence, incurred sample reanalysis

This content is open access.

Palabras clave
nitrofurantoin, LC-MS/MS, solid phase extraction, human plasma, bioequivalence, incurred sample reanalysis