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Adaptive morphofunctional rearrangements in the adult rats adenohypophysis after long-term exposure of heavy metal salts

Hryntsova Nataliia
Hryntsova Nataliia
, 
Hodorova Ingrid
Hodorova Ingrid
, 
Romanyuk Anatoly
Romanyuk Anatoly
, 
Kravets Alexander
Kravets Alexander
, 
Rybarova Silvia
Rybarova Silvia
, 
Lovasova Kvetuse
Lovasova Kvetuse
, 
Mihalik Josef
Mihalik Josef
, 
Pavliuk-Karachevtseva Andriana
Pavliuk-Karachevtseva Andriana
, 
Vecanova Janka
Vecanova Janka
, 
Kolesar Dalibor
Kolesar Dalibor
, 
Bolekova Andriana
Bolekova Andriana
 y 
Flesarova Slavka
Flesarova Slavka
   | 23 ago 2022
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Publicado en línea: 23 ago 2022
Páginas: 70 - 78
Recibido: 17 may 2022
Aceptado: 12 jun 2022
DOI: https://doi.org/10.2478/acm-2022-0009
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© 2022 Hryntsova N. et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig.1

Morphological rearrangements in the experimental rat pituitary gland under the condition of 30-days adaptation to heavy metals salts: 1 – venous pleural effusion; 2 – edema tissue trabeculae; 3-collagenization of the vessel walls. Staining: A – by hematoxylin-eosin; B – by Mason-Goldner.
Morphological rearrangements in the experimental rat pituitary gland under the condition of 30-days adaptation to heavy metals salts: 1 – venous pleural effusion; 2 – edema tissue trabeculae; 3-collagenization of the vessel walls. Staining: A – by hematoxylin-eosin; B – by Mason-Goldner.

Fig. 2

Morphological rearrangements in the experimental rats pituitary gland under the condition of 30-days adaptation to heavy metals salts: 1 – edema of connective tissue trabeculae; 2 – discomplexation of cellular trabeculae; 3 – cyst. Staining: by hematoxylin-eosin.
Morphological rearrangements in the experimental rats pituitary gland under the condition of 30-days adaptation to heavy metals salts: 1 – edema of connective tissue trabeculae; 2 – discomplexation of cellular trabeculae; 3 – cyst. Staining: by hematoxylin-eosin.

Fig. 3

Cellular composition of the experimental rats adenohypophysis after the condition 2 of 30-day readaptation to heavy metal salts: 1 – chromophilic basophils; 2 – chromophobes; 3 – chromophilic acidophiles. Staining: by Mason Goldner.
Cellular composition of the experimental rats adenohypophysis after the condition 2 of 30-day readaptation to heavy metal salts: 1 – chromophilic basophils; 2 – chromophobes; 3 – chromophilic acidophiles. Staining: by Mason Goldner.

Fig.4

Expression of hsp90 in the cytoplasm of experimental animals adenocytes under the con dition of 30-day readaptation to 90-day exposure to heavy metal salts: 1 – high level of hsp90 expression in the adenocytes cytoplasm. Immunohistochemical study of hsp90 expression.
Expression of hsp90 in the cytoplasm of experimental animals adenocytes under the con dition of 30-day readaptation to 90-day exposure to heavy metal salts: 1 – high level of hsp90 expression in the adenocytes cytoplasm. Immunohistochemical study of hsp90 expression.

Indicators of experimental and control rats adenohypophysis after adaptation to long-term exposure to heavy metal salts complex (X ± SD).

Indicator Series of animals
Control rats (n=6) Experimental rats (n=6)
Pituitary length, mm 7.92±0.83 6.64±0.59
Pituitary width, mm 4.26±0.19 3.63±0.2*
Capsule thickness, μm 3.52±0.27 1.42±0.19***
Vessels area, μm2 215.83±5.96 224.3±7.96
Chromophobic cells 57.46 ± 2.58 65.15 ± 0.43 *
Chromophilic acidophiles 31.28 ± 1.71 30.2 ± 0.59
Chromophilic basophils 11.26 ± 0.06 4.65 ± 0.18 ***
ACTH (pg / ml) 591.0±1.83 319.5 ± 3.17***

Expression level of HSP90 in adenohypophysis adenocytes of control and experimental rats after adaptation to long-term exposure to heavy metal salts complex (X ± SD).

HSP90 expression Control group total number 6 animals Experimental group total number 6 animals
Low positive 1 69.05±0.16 –
Moderately positive 2 31.91±0.82 42.75±1.26***
Strong positive 3 – 57.14±1.61***
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Temas de la revista:
Medicine, Clinical Medicine, Internal Medicine, Cardiology
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