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<p>The study explore some of the factors showing the biocompatibility and cellular metabolism of stem cells from apical papilla after their treatment with hapten solutions of two intracanal medicaments - Hydrocal and Acroseal. The MTT test and Annexin V test are used and the levels of HMOX-1 and IDO monitored after 48 hours of incubation in hapten solutions. In addition to proliferation and apoptosis, the possible sensitizing potential of these dental materials is assessed. The results show that after 48 hours, the groups treated with dental haptens found significantly fewer living cells than in the control group, which is especially indicative in the case of Acroseal. The proliferative activity of stem cells is increased, but without significant differences. HMOX-1 was statistically elevated in the Acroseal-treated group, indicative of its sensitizing capacity. IDO is statistically elevated in the group of cells treated with Hydrocal, which unequivocally speaks of the serious oxidative stress up to 48 hours and the need for protective action of this enzyme.</p>
</abstract>

The study explore some of the factors showing the biocompatibility and cellular metabolism of stem cells from apical papilla after their treatment with hapten solutions of two intracanal medicaments - Hydrocal and Acroseal. The MTT test and Annexin V test are used and the levels of HMOX-1 and IDO monitored after 48 hours of incubation in hapten solutions. In addition to proliferation and apoptosis, the possible sensitizing potential of these dental materials is assessed. The results show that after 48 hours, the groups treated with dental haptens found significantly fewer living cells than in the control group, which is especially indicative in the case of Acroseal. The proliferative activity of stem cells is increased, but without significant differences. HMOX-1 was statistically elevated in the Acroseal-treated group, indicative of its sensitizing capacity. IDO is statistically elevated in the group of cells treated with Hydrocal, which unequivocally speaks of the serious oxidative stress up to 48 hours and the need for protective action of this enzyme.

In vitro evaluation of biocompatibility of two intracanal medicaments on stem cells from apical papilla – a comparative study

Department of Imaging and Oral Diagnostics, Faculty of Dental Medicine

Department of Conservative Dentistry, Faculty of Dental Medicine

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The study explore some of the factors showing the biocompatibility and cellular metabolism of stem cells from apical papilla after their treatment with hapten...

In vitro evaluation of biocompatibility of two intracanal medicaments on stem cells from apical papilla – a comparative study

Publicado en línea: 30 sept 2022

Páginas: 83 - 88

Recibido: 23 jun 2022

Aceptado: 26 ago 2022

DOI: https://doi.org/10.2478/acb-2022-0013

Palabras clave
intracanal medicaments, calcium hydroxide, epoxy based sealer, dental haptens, apoptosis

© 2022 Maria Dencheva et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

In vitro evaluation of biocompatibility of two intracanal medicaments on stem cells from apical papilla – a comparative study

Publicado en línea: 30 sept 2022

Páginas: 83 - 88

Recibido: 23 jun 2022

Aceptado: 26 ago 2022

DOI: https://doi.org/10.2478/acb-2022-0013

Palabras claveintracanal medicaments, calcium hydroxide, epoxy based sealer, dental haptens, apoptosis

© 2022 Maria Dencheva et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
intracanal medicaments, calcium hydroxide, epoxy based sealer, dental haptens, apoptosis