<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Heart failure (HF) is one of the main causes of death worldwide. Recent studies reported altered DNA methylation in failing human hearts. This may suggest a role of DNA methylation, most frequently implicated in epigenetic control, in the development of heart failure. Here, employing RT-qPCR, we characterized transcript levels for main DNA methyltransferases (DNMTs), DNMT1, DNMT3A, and DNMT3B, mediate DNA methylation, and they have different functions that complement each other during methylation. All analyzes were performed at different stages of porcine myocardial cell primary culture. In the present study we demonstrated increasing transcript expression levels for all analyzed genes during <italic>in vitro</italic> cultivation. The changes for DNMT1 and DNMT3A seem to be particularly important, where statistically significant changes were observed.</p><p><bold>Running title:</bold> DNMTs role in cardiac muscle cell culture</p></abstract>

Heart failure (HF) is one of the main causes of death worldwide. Recent studies reported altered DNA methylation in failing human hearts. This may suggest a role of DNA methylation, most frequently implicated in epigenetic control, in the development of heart failure. Here, employing RT-qPCR, we characterized transcript levels for main DNA methyltransferases (DNMTs), DNMT1, DNMT3A, and DNMT3B, mediate DNA methylation, and they have different functions that complement each other during methylation. All analyzes were performed at different stages of porcine myocardial cell primary culture. In the present study we demonstrated increasing transcript expression levels for all analyzed genes during in vitro cultivation. The changes for DNMT1 and DNMT3A seem to be particularly important, where statistically significant changes were observed.Running title: DNMTs role in cardiac muscle cell culture

Increased transcript expression levels of DNA methyltransferases type 1 and 3A during cardiac muscle long-term cell culture

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology

Department of Cardiac Surgery and Transplantology

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Heart failure (HF) is one of the main causes of death worldwide. Recent studies reported altered DNA methylation in failing human hearts. This may suggest a...

GENE	PRIMER SEQUENCE (5′–3′)		PRODUCT SIZE (BP)
DNMT1	F	GTGAGGACATGCAGCTTTCA	211
DNMT1	R	AACTTGTTGTCCTCCGTTGG	211
DNMT3A	F	CTGAGAAGCCCAAGGTCAAG	238
DNMT3A	R	CAGCAGATGGTGCAGTAGGA	238
DNMT3B	F	ACAGGTCGGCTCTTCTTTGA	245
DNMT3B	R	AAAGCCCCTCGTTACCTGTT	245

Increased transcript expression levels of DNA methyltransferases type 1 and 3A during cardiac muscle long-term cell culture

Publicado en línea: 30 mar 2021

Páginas: 27 - 32

Recibido: 05 feb 2021

Aceptado: 03 mar 2021

DOI: https://doi.org/10.2478/acb-2021-0005

Palabras clave
epigenetics, DNMTs, cardiac muscle, RT-qPCR

© 2021 Mariusz J. Nawrocki et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Figure 2

Oligonucleotide sequences of primers used for RT-qPCR analysis

Increased transcript expression levels of DNA methyltransferases type 1 and 3A during cardiac muscle long-term cell culture

Publicado en línea: 30 mar 2021

Páginas: 27 - 32

Recibido: 05 feb 2021

Aceptado: 03 mar 2021

DOI: https://doi.org/10.2478/acb-2021-0005

Palabras claveepigenetics, DNMTs, cardiac muscle, RT-qPCR

© 2021 Mariusz J. Nawrocki et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Figure 2

Oligonucleotide sequences of primers used for RT-qPCR analysis

Palabras clave
epigenetics, DNMTs, cardiac muscle, RT-qPCR