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Copy number variations of cytochrome P450 genes in Kinh Vietnamese

Nhung Phuong Vu

Nhung Phuong Vu

  • Department of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and TechnologyHanoi, Vietnam
  • Genome Analysis Laboratory, Institute of Genome Research, Vietnam Academy of Science and TechnologyHanoi, Vietnam
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Vu, Nhung Phuong
, 
Ton Dang Nguyen

Ton Dang Nguyen

  • Department of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and TechnologyHanoi, Vietnam
  • Genome Analysis Laboratory, Institute of Genome Research, Vietnam Academy of Science and TechnologyHanoi, Vietnam
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Nguyen, Ton Dang
, 
Binh Huy Nguyen

Binh Huy Nguyen

Department of Physiology, Hanoi Medical UniversityHanoi, Vietnam
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Nguyen, Binh Huy
, 
Duong Thuy Nguyen

Duong Thuy Nguyen

  • Department of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and TechnologyHanoi, Vietnam
  • Genome Analysis Laboratory, Institute of Genome Research, Vietnam Academy of Science and TechnologyHanoi, Vietnam
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Nguyen, Duong Thuy
, 
Hai Van Nong

Hai Van Nong

  • Department of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and TechnologyHanoi, Vietnam
  • Genome Analysis Laboratory, Institute of Genome Research, Vietnam Academy of Science and TechnologyHanoi, Vietnam
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Nong, Hai Van
 y 
Ha Hai Nguyen

Ha Hai Nguyen

  • Department of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and TechnologyHanoi, Vietnam
  • Genome Analysis Laboratory, Institute of Genome Research, Vietnam Academy of Science and TechnologyHanoi, Vietnam
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Nguyen, Ha Hai
  
17 sept 2023
Copy number variations of cytochrome P450 genes in Kinh Vietnamese's Cover Image
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Categoría del artículo: Brief communication
Publicado en línea: 17 sept 2023
Páginas: 84 - 92
DOI: https://doi.org/10.2478/abm-2023-0048
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© 2023 Nhung Phuong Vu et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Background

The cytochrome P450 (CYP450) family is well known as a major group of drug metabolizing enzymes. The polymorphism of CYP450 genes is the main factor having an impact on the interindividual difference in drug response, including drug efficacy and drug safety. The single nucleotide polymorphism (SNPs) of Vietnamese Kinh has been widely studied, but information about the copy number variations (CNVs) of other CYP450 genes is still unknown.

Objective

To identify the CNV variability of CYP450 in 154 healthy unrelated Kinh Vietnamese, except eCYP2D6, which was previously reported.

Methods

Multiplex Ligation-Dependent Probe Amplification (MLPA) was applied for determination of copy number of 10 CYP450 genes. Later, PCR or quantitative PCR (qPCR) was used to confirm the detected CNVs in randomly chosen subjects.

Results

Of the 154 subjects, along with CYP2D6, 4 other CYP450 genes showed CNVs including duplications (CYP1B1), deletions (CYP2A6 and CYP2C9), and both duplications and deletions (CYP2E1). Among these, CYP2A6 exhibited the greatest frequency of CNVs compared with other CYP450, in which CYP2A6Del accounted for 11%. Meanwhile, allele CYP2E1Del showed the lowest frequency with only 0.3%.

Conclusions

The present study provides new insight into CYP450 CNVs in the Kinh Vietnamese cohort. Our data have contributed to genetic profiling of CYP450 CNVs in Vietnam, which would be helpful for facilitating implementation of pharmacogenetics in drug dosing adjustment in Vietnam.
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Inglés
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Temas de la revista:
Medicina, Profesiones auxiliares, enfermería, Ciencias médicas básicas, Ciencias médicas básicas, otros, Medicina Clínica, Medicina Clínica, otros
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