<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Background</h3><p>Breast cancer represents the second most deadly malignancy in women, and long noncoding RNAs (lncRNAs) have crucial functions in its development.</p></sec>
<sec><h3>Objective</h3><p>To investigate effects of the promoter of <italic>CDKN1A</italic> antisense DNA damage-activated RNA (<italic>PANDAR</italic>) on epithelial-mesenchymal transition (EMT) in breast cancer cells and their proliferation.</p></sec>
<sec><h3>Methods</h3><p>lncRNAs potentially regulating the transcriptional activity of the E-cadherin (E-cad, an epithelial cell marker) gene promoter were screened using a dual-luciferase reporter assay. <italic>PANDAR</italic> was overexpressed in Michigan cancer foundation 7 (MCF-7) breast cancer cells. E-cad and N-cadherin (N-cad, a mesenchymal cell marker) levels were detected by immunoblotting. Cell viability was assessed using a cell counting kit-8.</p></sec>
<sec><h3>Results</h3><p><italic>PANDAR</italic> and <italic>TCONS00068220</italic>/<italic>LOC105375819</italic> conservatively regulated the promoter activity of <italic>E-cad</italic>. <italic>PANDAR</italic> overexpression in MCF-7 inhibited E-cad expression, but upregulated N-cad. The enhanced expression of <italic>PANDAR</italic> promoted cell proliferation.</p></sec>
<sec><h3>Conclusion</h3><p><italic>PANDAR</italic> is a key transcriptional repressor of <italic>E-cad</italic> and has regulatory effects on the promotion of cell proliferation. <italic>PANDAR</italic> is an oncogene in breast cancer, potentially facilitating the EMT process and promoting cell proliferation.</p></sec>
</abstract>

BackgroundBreast cancer represents the second most deadly malignancy in women, and long noncoding RNAs (lncRNAs) have crucial functions in its development.
ObjectiveTo investigate effects of the promoter of CDKN1A antisense DNA damage-activated RNA (PANDAR) on epithelial-mesenchymal transition (EMT) in breast cancer cells and their proliferation.
MethodslncRNAs potentially regulating the transcriptional activity of the E-cadherin (E-cad, an epithelial cell marker) gene promoter were screened using a dual-luciferase reporter assay. PANDAR was overexpressed in Michigan cancer foundation 7 (MCF-7) breast cancer cells. E-cad and N-cadherin (N-cad, a mesenchymal cell marker) levels were detected by immunoblotting. Cell viability was assessed using a cell counting kit-8.
ResultsPANDAR and TCONS00068220/LOC105375819 conservatively regulated the promoter activity of E-cad. PANDAR overexpression in MCF-7 inhibited E-cad expression, but upregulated N-cad. The enhanced expression of PANDAR promoted cell proliferation.
ConclusionPANDAR is a key transcriptional repressor of E-cad and has regulatory effects on the promotion of cell proliferation. PANDAR is an oncogene in breast cancer, potentially facilitating the EMT process and promoting cell proliferation.

Regulatory roles of lncRNA <italic>PANDAR</italic> in breast cancer cell proliferation

Inner Mongolia Cancer Hospital and Affiliated People's Hospital of Inner Mongolia Medical University

Affiliated People's Hospital of Inner Mongolia Medical University

Asian Biomedicine

This work is licensed under the Creative Commons Attribution 4.0 International License.

Regulatory roles of lncRNA  in breast cancer cell proliferation

BackgroundBreast cancer represents the second most deadly malignancy in women, and long noncoding RNAs (lncRNAs) have crucial functions in its...

Regulatory roles of lncRNA PANDAR in breast cancer cell proliferation

Article Category: Brief communication (original)

Publicado en línea: 30 dic 2021

Páginas: 285 - 291

DOI: https://doi.org/10.2478/abm-2021-0035

Palabras clave
breast neoplasms, epithelial-mesenchymal transition, long noncoding RNA PANDAR, human, cell proliferation

© 2021 Qinnuan Sun et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Regulatory roles of lncRNA PANDAR in breast cancer cell proliferation

Article Category: Brief communication (original)

Publicado en línea: 30 dic 2021

Páginas: 285 - 291

DOI: https://doi.org/10.2478/abm-2021-0035

Palabras clavebreast neoplasms, epithelial-mesenchymal transition, long noncoding RNA PANDAR, human, cell proliferation

© 2021 Qinnuan Sun et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Palabras clave
breast neoplasms, epithelial-mesenchymal transition, long noncoding RNA PANDAR, human, cell proliferation