<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Gastric cancer is one of the most common cancers of the gastrointestinal system, and its overall five-year survival rate is still 15 % to 20 %, as it can mostly be diagnosed at an advanced stage. On the other hand, although colorectal cancer has a rather good prognosis, mortality is one half that of the incidence.</p><p>As carcinogenesis is believed to involve reactive radicals that cause DNA adduct formation, impaired repair activity, and weakened tumour suppression, it would help to understand the role of the polymorphisms of nucleotide excision repair enzyme XRCC1 and of tumour suppressor gene <italic>p53</italic> in gastric and colorectal cancers. Our study included 94 gastric cancer patients, 96 colorectal cancer patients, and 108 cancer-free individuals as control with the aim to see if there was an association between <italic>XRCC1</italic> Arg399Gln and <italic>p53</italic> Arg72Pro polymorphisms and cancer susceptibility. DNA was extracted from peripheral blood cells and genotypes were determined using the polymerase chain reaction-restriction fragment length polymorphism. Polymorphism <italic>p53</italic> Arg72Pro was not associated with either gastric or colorectal carcinoma, while <italic>XRCC1</italic> Arg399Gln was not associated with the increased risk of colorectal cancer. However, <italic>XRCC1</italic> homozygous Gln allele at codon 399 was associated with 2.54 times higher risk of gastric cancer.</p></abstract>

Gastric cancer is one of the most common cancers of the gastrointestinal system, and its overall five-year survival rate is still 15 % to 20 %, as it can mostly be diagnosed at an advanced stage. On the other hand, although colorectal cancer has a rather good prognosis, mortality is one half that of the incidence.As carcinogenesis is believed to involve reactive radicals that cause DNA adduct formation, impaired repair activity, and weakened tumour suppression, it would help to understand the role of the polymorphisms of nucleotide excision repair enzyme XRCC1 and of tumour suppressor gene p53 in gastric and colorectal cancers. Our study included 94 gastric cancer patients, 96 colorectal cancer patients, and 108 cancer-free individuals as control with the aim to see if there was an association between XRCC1 Arg399Gln and p53 Arg72Pro polymorphisms and cancer susceptibility. DNA was extracted from peripheral blood cells and genotypes were determined using the polymerase chain reaction-restriction fragment length polymorphism. Polymorphism p53 Arg72Pro was not associated with either gastric or colorectal carcinoma, while XRCC1 Arg399Gln was not associated with the increased risk of colorectal cancer. However, XRCC1 homozygous Gln allele at codon 399 was associated with 2.54 times higher risk of gastric cancer.

Association Between <italic>XRCC1</italic> ARG399GLN and <italic>P53</italic> ARG72PRO Polymorphisms and the Risk of Gastric and Colorectal Cancer in Turkish Population

Faculty of Pharmacy Department of Toxicology, Gazi University, Ankara, Turkey

Faculty of Medicine, Department of General Surgery, Beşevler, Gazi University, Ankara, Turkey

Archives of Industrial Hygiene and Toxicology

Association Between  ARG399GLN and  ARG72PRO Polymorphisms and the Risk of Gastric and Colorectal Cancer in Turkish Population

Gastric cancer is one of the most common cancers of the gastrointestinal system, and its overall five-year survival rate is still 15 % to 20 %, as it can...

Association Between XRCC1 ARG399GLN and P53 ARG72PRO Polymorphisms and the Risk of Gastric and Colorectal Cancer in Turkish Population

Publicado en línea: 04 oct 2011

Páginas: 207 - 214

DOI: https://doi.org/10.2478/10004-1254-62-2011-2098

Palabras clave
DNA-adukt, interakcija između gena, PCR-RFLP

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