<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Human nerve growth factor β (β-NGF) is considered a major therapeutic agent for treatment of neurodegenerative diseases. We have previously reported the optimized conditions for β-NGF overproduction in <italic>Escherichia coli</italic> in a shake-flask culture. In this study the optimal %DO (dissolved oxygen) and post induction temperature values for improved production of β-NGF were found in the bioreactor scale using response surface methodology (RSM) as the most common statistical method. Also, for further enhancement of the yield, different post-induction periods of time were selected for testing. In all experiments, the productivity level and bacterial cell growth were evaluated by western blotting technique and monitoring of absorbance at 600 nm, respectively. Our results indicated that %DO, the post-induction time and temperature have significant effects on the production of β-NGF. After 2 hours of induction, the low post induction temperature of 32°C and 20% DO were used to increase the production of β-NGF in a 5-l bioreactor. Another important result obtained in this study was that the improved β-NGF production was not achieved at highest dry cell weigh or highest cell growth. These results are definitely of importance for industrial β-NGF production.</p></abstract>

Human nerve growth factor β (β-NGF) is considered a major therapeutic agent for treatment of neurodegenerative diseases. We have previously reported the optimized conditions for β-NGF overproduction in Escherichia coli in a shake-flask culture. In this study the optimal %DO (dissolved oxygen) and post induction temperature values for improved production of β-NGF were found in the bioreactor scale using response surface methodology (RSM) as the most common statistical method. Also, for further enhancement of the yield, different post-induction periods of time were selected for testing. In all experiments, the productivity level and bacterial cell growth were evaluated by western blotting technique and monitoring of absorbance at 600 nm, respectively. Our results indicated that %DO, the post-induction time and temperature have significant effects on the production of β-NGF. After 2 hours of induction, the low post induction temperature of 32°C and 20% DO were used to increase the production of β-NGF in a 5-l bioreactor. Another important result obtained in this study was that the improved β-NGF production was not achieved at highest dry cell weigh or highest cell growth. These results are definitely of importance for industrial β-NGF production.

Improved Production of Recombinant Human β-NGF in <italic>Escherichia coli</italic> – a Bioreactor Scale Study

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Improved Production of Recombinant Human β-NGF in  – a Bioreactor Scale Study

Human nerve growth factor β (β-NGF) is considered a major therapeutic agent for treatment of neurodegenerative diseases. We have previously reported the...

Improved Production of Recombinant Human β-NGF in Escherichia coli – a Bioreactor Scale Study

Article Category: original-paper

Publicado en línea: 04 sept 2018

Páginas: 355 - 363

Recibido: 22 mar 2018

Aceptado: 16 jun 2018

DOI: https://doi.org/10.21307/pjm-2018-045

Palabras clave
bioreactor, RSM, β-NGF over production,

© 2018 Zahra Hajihassan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Improved Production of Recombinant Human β-NGF in Escherichia coli – a Bioreactor Scale Study

Article Category: original-paper

Publicado en línea: 04 sept 2018

Páginas: 355 - 363

Recibido: 22 mar 2018

Aceptado: 16 jun 2018

DOI: https://doi.org/10.21307/pjm-2018-045

Palabras clavebioreactor, RSM, β-NGF over production,

© 2018 Zahra Hajihassan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
bioreactor, RSM, β-NGF over production,