Acceso abierto

Morphological and molecular characterization of Bitylenchus hispaniensis (Nematoda: Telotylenchidae) from Iran

Abbas Abdolkhani
Abbas Abdolkhani
 y 
Sedighe Azimi
Sedighe Azimi
   | 03 nov 2021
Journal of Nematology's Cover Image
Acerca de este artículo
Artículo anterior
Artículo siguiente
Cite
Publicado en línea: 03 nov 2021
Páginas: 1 - 10
DOI: https://doi.org/10.21307/jofnem-2021-092
Palabras clave
© 2021 Abbas Abdolkhani et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Line drawings of Bitylenchus hispaniensis from Iran. A-F: Female. A: Anterior body region; B: Lateral field at mid-body; C: Part of reproductive system; D-F: Posterior body region; G, H: Posterior body region of male.
Line drawings of Bitylenchus hispaniensis from Iran. A-F: Female. A: Anterior body region; B: Lateral field at mid-body; C: Part of reproductive system; D-F: Posterior body region; G, H: Posterior body region of male.

Figure 2:

Light photomicrographs of Bitylenchus hispaniensis from Iran. A, C-M: Female. A: Entire body; C, D: Anterior body region; E-G: Pharyngeal region; H: Lateral field at mid-body; I, J: Vulval region; K-M: Posterior body region (the arrow indicates the phasmid); B, N-P: Male. B: Entire body; N-P: Posterior body region. (Scale bars: A, B = 50 μm; C-P = 10 μm).
Light photomicrographs of Bitylenchus hispaniensis from Iran. A, C-M: Female. A: Entire body; C, D: Anterior body region; E-G: Pharyngeal region; H: Lateral field at mid-body; I, J: Vulval region; K-M: Posterior body region (the arrow indicates the phasmid); B, N-P: Male. B: Entire body; N-P: Posterior body region. (Scale bars: A, B = 50 μm; C-P = 10 μm).

Figure 3:

Bayesian 50% majority rule consensus tree inferred from analysis of the D2-D3 domains of the LSU rDNA sequences of Iranian population of Bitylenchus hispaniensis under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the D2-D3 domains of the LSU rDNA sequences of Iranian population of Bitylenchus hispaniensis under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequences are indicated in bold.

Figure 4:

Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rRNA gene of Iranian population of Bitylenchus hispaniensis under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequence is indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the ITS rRNA gene of Iranian population of Bitylenchus hispaniensis under the GTR + G + I model. Bayesian posterior probability values of more than 0.50 are given for appropriate clades. New sequence is indicated in bold.

Morphometrics of Bitylenchus hispaniensis from Khuzestan province, Iran and compare with the original description and Greek population.

Khuzestan province, Iran Handoo et al. (2014) Córdoba, Córdoba, Spain Tzortzakakis et al. (2018) Crete, Greece
Character Females Males Females Males Females
n 14 9 7 10 3
L 622.1 ± 41.0 (570–704) 661.7 ± 58.0 (591–763) 662 ± 38.4 (612–732) 640 ± 60.9 (548–760) 722 ± 47 (670–760)
a 25.6 ± 3.5 (21.8–2.8) 29.5 ± 0.1 (27.6–32.8) 30.4 ± 1.7 (27.8–32.5) 31.0 ± 3.9 (27.0–39.1) 32.3 ± 0.6 (31.9–33.0)
b 5.5 ± 0.4 (5.0–6.3) 5.9 ± 0.4 (5.3–6.5) 6.0 ± 0.4 (5.6–6.8) 6.1 ± 0.7 (5.4–7.8) 6.3 ± 0.4 (6.1–6.8)
c 13.3 ± 0.7 (12.8–15.7) 14.6 ± 0.7 (13.5–15.7) 14.2 ± 1.7 (11.8–16.8) 17.7 ± 1.4 (15.2–20.7) 14.9 ± 0.6 (14.4–15.5)
c′ 2.8 ± 0.4 (2.5–3.2) 2.9 ± 0.2 (2.6–3.2) 2.9 ± 0.3 (2.4–3.3) 2.7 ± 0.2 (2.5–3.0) 3.0 ± 0.2 (2.7–3.2)
V 54.3 ± 1.5 (52.7–57.5) - 54.6 ± 1.5 (52.5–57.0) - 55.0 ± 1.0 (54–56)
Lip region height 3.7 ± 0.9 (3.2–4.1) 3.4 ± 0.3 (3.2–4.1) 3.6 ± 0.3 (3–4) - -
Lip region width 6.7 ± 0.2 (6.5–7.0) 6.4 ± 0.1 (6.0–6.5) 7.2 ± 0.3 (6.5–7.5) - -
Stylet length 17.5 ± 0.3 (17–18) 17.1 ± 0.4 (16.2–17.5) 16.1 ± 0.5 (15.5–17.0) 15.9 ± 0.8 (15.0–18.0) 17.3 ± 0.3 (17.0–17.5)
m 58.0 ± 2.7 (55.7–63.1) 54.2 ± 2.7 (48.2–57.3) - - -
DGO 2.9 ± 0.2 (2.6–3.0) 2.8 ± 0.2 (2.5–3.0) 2.5 - -
MB 49.2 ± 2.1 (46.8–52.6) 51.2 ± 4.0 (45.2–57.3) - - -
Maximum body width 24.7 ± 7.4 (20.8–29.2) 20.8 ± 1.3 (18.8–23.4) 21.8 ± 1.0 (20.5–23.5) 20.7 ± 1.3 (18.0–23.0) 22.3 ± 1.2 (21–23)
Pharynx length 115.3 ± 6.7 (105–120) 116.0 ± 6.8 (106–124) 110.0 ± 6.5 (100–120) 105.4 ± 8.2 (90–120) 114.0 ± 5.3 (110–120)
Anterior end to median bulb 56.7 ± 3.8 (50.0–61.7) 56.9 ± 2.8 (52–58) - - -
Anterior end to nerve ring 77.8 ± 2.9 (73.0–84.5) 80.0 ± 4.2 (75.0–87.7) 83.1 ± 5.6 (73–95) - -
Anterior end to excretory pore 93.3 ± 6.1 (85.5–104.0) 87.6 ± 9.0 (87–104) 89.7 ± 3.9 (85–95) - -
Body width at vulva 24.5 ± 2.7 (21.5–27.9) - - -
Anal body width 16.5 ± 1.5 (15.0–19.5) 15.4 ± 1.5 (13.6–17.5) 16.1 ± 1.4 (13.5–17.5) 12.8 ± 0.8 (12–15) 16.5 ± 2.2 (14–18)
Tail length 47.4 ± 6.5 (37.0–55.2) 43.9 ± 3.5 (39.5–48.0) 47.4 ± 6.3 (37.5–57.0) 36.6 ± 5.6 (30–50) 48.3 ± 3.1 (45–51)
Tail annuli 51.8 ± 5.3 (40–58) - 55.1 ± 8.1 (40–66) - -
Spicule length - 25.5 ± 0.8 (24.0–26.5) - 25.3 ± 1.8 (23–30) -
Gubernaculum length - 12.5 ± 0.4 (12.0–13.3) - 11.4 ± 1.6 (10–15) -
eISSN:
2640-396X
Idioma:
Inglés
Calendario de la edición:
Volume Open
Temas de la revista:
Life Sciences, other
RSS Feed de revista