<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The study of species biodiversity within the <italic>Caenorhabditis</italic> genus of nematodes would be facilitated by the isolation of as many species as possible. So far, over 50 species have been found, usually associated with decaying vegetation or soil samples, with many from Africa, South America and Southeast Asia. Scientists based in these regions can contribute to <italic>Caenorhabditis</italic> sampling and their proximity would allow intensive sampling, which would be useful for understanding the natural history of these species. However, severely limited research budgets are often a constraint for these local scientists. In this study, we aimed to find a more economical, alternative growth media to rear <italic>Caenorhabditis</italic> and related species. We tested 25 media permutations using cheaper substitutes for the reagents found in the standard nematode growth media (NGM) and found three media combinations that performed comparably to NGM with respect to the reproduction and longevity of <italic>C. elegans</italic>. These new media should facilitate the isolation and characterization of <italic>Caenorhabditis</italic> and other free-living nematodes for the researchers in the poorer regions such as Africa, South America, and Southeast Asia where nematode diversity appears high.</p>
</abstract>

The study of species biodiversity within the Caenorhabditis genus of nematodes would be facilitated by the isolation of as many species as possible. So far, over 50 species have been found, usually associated with decaying vegetation or soil samples, with many from Africa, South America and Southeast Asia. Scientists based in these regions can contribute to Caenorhabditis sampling and their proximity would allow intensive sampling, which would be useful for understanding the natural history of these species. However, severely limited research budgets are often a constraint for these local scientists. In this study, we aimed to find a more economical, alternative growth media to rear Caenorhabditis and related species. We tested 25 media permutations using cheaper substitutes for the reagents found in the standard nematode growth media (NGM) and found three media combinations that performed comparably to NGM with respect to the reproduction and longevity of C. elegans. These new media should facilitate the isolation and characterization of Caenorhabditis and other free-living nematodes for the researchers in the poorer regions such as Africa, South America, and Southeast Asia where nematode diversity appears high.

<article-title xml:lang="en">Cultivation of <italic>Caenorhabditis elegans</italic> on new cheap monoxenic media without peptone

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Cultivation of  on new cheap monoxenic media without peptone

The study of species biodiversity within the Caenorhabditis genus of nematodes would be facilitated by the isolation of as many species as possible. So far,...

Cultivation of Caenorhabditis elegans on new cheap monoxenic media without peptone

Publicado en línea: 01 ene 2021

Páginas: 1 - 23

DOI: https://doi.org/10.21307/jofnem-2021-036

Palabras clave
Growth media, Worm cultivation, Brood size, Lifespan

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Cultivation of Caenorhabditis elegans on new cheap monoxenic media without peptone

Publicado en línea: 01 ene 2021

Páginas: 1 - 23

DOI: https://doi.org/10.21307/jofnem-2021-036

Palabras claveGrowth media, Worm cultivation, Brood size, Lifespan

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
Growth media, Worm cultivation, Brood size, Lifespan