<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Sampling for needle nematodes was carried out in a grapevine area in Thessaloniki, North Greece and two nematode species of <italic>Longidorus</italic> (<italic>L. pauli</italic> and <italic>L. pisi</italic>) were collected. Nematodes were extracted from 500 cm<sup>3</sup> of soil by modified sieving and decanting method, processed to glycerol and mounted on permanent slides, and subsequently identified morphologically and molecularly. Nematode DNA was extracted from single individuals and PCR assays were conducted to amplify D2-D3 expansion segments of 28S rRNA, ITS1 rRNA, and partial mitochondrial coxI regions. Morphology and morphometry data obtained from these populations were consistent with <italic>L. pauli</italic> and <italic>L. pisi</italic> identifications. To our knowledge, this is the first report of <italic>L. pauli</italic> for Greece, and the second world report after the original description from Idleb, Syria, extending the geographical distribution of this species in the Mediterranean Basin.</p>
</abstract>

Sampling for needle nematodes was carried out in a grapevine area in Thessaloniki, North Greece and two nematode species of Longidorus (L. pauli and L. pisi) were collected. Nematodes were extracted from 500 cm3 of soil by modified sieving and decanting method, processed to glycerol and mounted on permanent slides, and subsequently identified morphologically and molecularly. Nematode DNA was extracted from single individuals and PCR assays were conducted to amplify D2-D3 expansion segments of 28S rRNA, ITS1 rRNA, and partial mitochondrial coxI regions. Morphology and morphometry data obtained from these populations were consistent with L. pauli and L. pisi identifications. To our knowledge, this is the first report of L. pauli for Greece, and the second world report after the original description from Idleb, Syria, extending the geographical distribution of this species in the Mediterranean Basin.

<article-title xml:lang="en">Morphological and molecular characterisation of <italic>Longidorus pauli</italic> (Nematoda: Longidoridae), first report from Greece

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Morphological and molecular characterisation of  (Nematoda: Longidoridae), first report from Greece

Sampling for needle nematodes was carried out in a grapevine area in Thessaloniki, North Greece and two nematode species of Longidorus (L. pauli and L. pisi)...

Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece

Publicado en línea: 01 ene 2021

Páginas: 1 - 10

DOI: https://doi.org/10.21307/jofnem-2021-034

Palabras clave
Cytochrome oxidase c subunit 1, D2-D3 of 28S rRNA, Description, ITS1 rRNA, Needle nematodes, Taxonomy

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece

Publicado en línea: 01 ene 2021

Páginas: 1 - 10

DOI: https://doi.org/10.21307/jofnem-2021-034

Palabras claveCytochrome oxidase c subunit 1, D2-D3 of 28S rRNA, Description, ITS1 rRNA, Needle nematodes, Taxonomy

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
Cytochrome oxidase c subunit 1, D2-D3 of 28S rRNA, Description, ITS1 rRNA, Needle nematodes, Taxonomy