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First report on molecular identification of Anisakis simplex in Oncorhynchus nerka from the fish market, with taxonomical issues within Anisakidae

Alina E. Safonova

Alina E. Safonova

Far Eastern Federal UniversityVladivostok, Russia
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Safonova, Alina E.
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Anastasia N. Voronova

Anastasia N. Voronova

Federal Scientific Center of the East Asia Terrestrial Biodiversity FEB RASVladivostok, Russia
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Voronova, Anastasia N.
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Konstantin S. Vainutis

Konstantin S. Vainutis

Federal Scientific Center of the East Asia Terrestrial Biodiversity FEB RASVladivostok, Russia
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Vainutis, Konstantin S.
  
01 ene 2021
First report on molecular identification of Anisakis simplex in Oncorhynchus nerka from the fish market, with taxonomical issues within Anisakidae's Cover Image
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Publicado en línea: 01 ene 2021
Páginas: 1 - 10
DOI: https://doi.org/10.21307/jofnem-2021-023
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© 2021 Authors, published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Phylogenetic relationships of family Anisakidae based on ITS1-5.8S-ITS2 rDNA sequences reconstructed by Maximum Likelihood (ML) and Neighbor-Joining (NJ) methods. Nodal support values are shown based on 1,000 bootstrap replicates (ML/NJ). The tree was drawn to scale, with branch lengths measured in the number of substitutions per site. Accession numbers are given for each species/strain at the end of each sequence. The arrows with generic names are indicating branches. Genetic distances between species and genera are indicated at the junction of the dotted lines. The scale bar represents the number of substitutions per site.
Phylogenetic relationships of family Anisakidae based on ITS1-5.8S-ITS2 rDNA sequences reconstructed by Maximum Likelihood (ML) and Neighbor-Joining (NJ) methods. Nodal support values are shown based on 1,000 bootstrap replicates (ML/NJ). The tree was drawn to scale, with branch lengths measured in the number of substitutions per site. Accession numbers are given for each species/strain at the end of each sequence. The arrows with generic names are indicating branches. Genetic distances between species and genera are indicated at the junction of the dotted lines. The scale bar represents the number of substitutions per site.

Sequences of the ITS1-5_8S-ITS2 rDNA region from GenBank used in this study_

Accession number Species Geographical region
EU718471 Anisakis simplex Morocco
JQ934875 Croatia
KJ011481 South Korea
KM273043 Denmark
KP645361 Poland
MF668920 USA
MN726484 Japan
GQ169364 Ireland
MH211473 A. pegreffii China
KF032056 A. simplex × A. pegreffii
KF032058 Turkey
KF032060
KY524216 A. berlandi Indonesia
JQ912692 A. nascettii Italy
JX486104 Brazil
JN005767 A. ziphidarum Portugal
EU718473 Mauritius
KF673776 A. typica China
EU327689 Brazil
KX098561 USA
JQ912694 A. brevispiculata Italy
EU327691 A. physeteris Brazil
GU295976 A. paggiae Greenland
KC970082 Pseudoterranova cattani Argentina
KM273078 P. decipiens Denmark
AB576757 P. azarasi Japan
KM491173 Contracaecum osculatum Denmark
EU678869 C. rudolphii Italy
JF424598 C. bioccai USA
LC422643 Ascaris lumbricoides Japan
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