<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Alive anisakids cause acute gastrointestinal diseases, and dead worms contained in food can provoke sensibilization and allergic reactions in humans. Detected in the purchased minced salmon <italic>Oncorhynchus nerka</italic> nematodes were identified as <italic>Anisakis simplex sensu stricto</italic> (Anisakidae). We found that recently published phylogenetic trees (reconstructed using different ribosomal and mitochondrial genetic markers) showed independent clusterization of species recognized in the <italic>A. simplex sensu lato</italic> species complex. This prompted us to undertake this full-fledged molecular genetics study of anisakids from Kamchatka with phylogenetic reconstructions (NJ/ML) and calculated ranges of interspecific and intergeneric p-distances using ITS1-5.8S-ITS2 sequences. We confirmed that molecular markers based on the ITS region of rDNA were able to recognize ‘pure’ specimens belonging to the cryptic species. We offer new insights into the systematics of anisakids. The genus <italic>Anisakis sensu stricto</italic> should include <italic>Anisakis simplex sensu stricto</italic>, <italic>Anisakis pegreffii</italic>, <italic>Anisakis berlandi</italic>, <italic>Anisakis ziphidarum</italic>, and <italic>Anisakis nascettii</italic>. Presumably, two genera should be restored in the structure of the subfamily Anisakinae: <italic>Skrjabinisakis</italic> for the species <italic>Anisakis paggiae</italic>, <italic>Anisakis brevispiculata</italic>, and <italic>Anisakis physeteris</italic>; and <italic>Peritrachelius</italic> for the species <italic>Anisakis typica</italic>. In addition, we provide the short annotated list of some genera of the family Anisakidae, including their diagnoses.</p>
</abstract>

Alive anisakids cause acute gastrointestinal diseases, and dead worms contained in food can provoke sensibilization and allergic reactions in humans. Detected in the purchased minced salmon Oncorhynchus nerka nematodes were identified as Anisakis simplex sensu stricto (Anisakidae). We found that recently published phylogenetic trees (reconstructed using different ribosomal and mitochondrial genetic markers) showed independent clusterization of species recognized in the A. simplex sensu lato species complex. This prompted us to undertake this full-fledged molecular genetics study of anisakids from Kamchatka with phylogenetic reconstructions (NJ/ML) and calculated ranges of interspecific and intergeneric p-distances using ITS1-5.8S-ITS2 sequences. We confirmed that molecular markers based on the ITS region of rDNA were able to recognize ‘pure’ specimens belonging to the cryptic species. We offer new insights into the systematics of anisakids. The genus Anisakis sensu stricto should include Anisakis simplex sensu stricto, Anisakis pegreffii, Anisakis berlandi, Anisakis ziphidarum, and Anisakis nascettii. Presumably, two genera should be restored in the structure of the subfamily Anisakinae: Skrjabinisakis for the species Anisakis paggiae, Anisakis brevispiculata, and Anisakis physeteris; and Peritrachelius for the species Anisakis typica. In addition, we provide the short annotated list of some genera of the family Anisakidae, including their diagnoses.

<article-title xml:lang="en">First report on molecular identification of <italic>Anisakis simplex</italic> in <italic>Oncorhynchus nerka</italic> from the fish market, with taxonomical issues within Anisakidae

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

First report on molecular identification of  in  from the fish market, with taxonomical issues within Anisakidae

Alive anisakids cause acute gastrointestinal diseases, and dead worms contained in food can provoke sensibilization and allergic reactions in humans. Detected...

Accession number	Species	Geographical region
EU718471	Anisakis simplex	Morocco
JQ934875		Croatia
KJ011481		South Korea
KM273043		Denmark
KP645361		Poland
MF668920		USA
MN726484		Japan
GQ169364		Ireland
MH211473	A. pegreffii	China
KF032056	A. simplex × A. pegreffii
KF032058		Turkey
KF032060
KY524216	A. berlandi	Indonesia
JQ912692	A. nascettii	Italy
JX486104		Brazil
JN005767	A. ziphidarum	Portugal
EU718473		Mauritius
KF673776	A. typica	China
EU327689		Brazil
KX098561		USA
JQ912694	A. brevispiculata	Italy
EU327691	A. physeteris	Brazil
GU295976	A. paggiae	Greenland
KC970082	Pseudoterranova cattani	Argentina
KM273078	P. decipiens	Denmark
AB576757	P. azarasi	Japan
KM491173	Contracaecum osculatum	Denmark
EU678869	C. rudolphii	Italy
JF424598	C. bioccai	USA
LC422643	Ascaris lumbricoides	Japan

First report on molecular identification of Anisakis simplex in Oncorhynchus nerka from the fish market, with taxonomical issues within Anisakidae

Publicado en línea: 01 ene 2021

Páginas: 1 - 10

DOI: https://doi.org/10.21307/jofnem-2021-023

Palabras clave
Detection, Anisakidosis, Systematics, Taxonomy, ITS,

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Sequences of the ITS1-5.8S-ITS2 rDNA region from GenBank used in this study.

First report on molecular identification of Anisakis simplex in Oncorhynchus nerka from the fish market, with taxonomical issues within Anisakidae

Publicado en línea: 01 ene 2021

Páginas: 1 - 10

DOI: https://doi.org/10.21307/jofnem-2021-023

Palabras claveDetection, Anisakidosis, Systematics, Taxonomy, ITS,

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Sequences of the ITS1-5.8S-ITS2 rDNA region from GenBank used in this study.

Palabras clave
Detection, Anisakidosis, Systematics, Taxonomy, ITS,