<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>
<italic>Rotylenchus wimbii</italic> n. sp. was found associated with finger millet in Kenya and is described based on light microscopy, scanning electron microscopy, and molecular information. Sequence analysis was performed on ITS, 18S, and D2-D3 of 28S of ribosomal DNA and <italic>COI</italic> of mitochondrial DNA. This new species is characterized by a moderate female body size of 0.6 to 0.8 mm, a continuous hemispherical lip region with four annuli, 3 to 4 irregular blocks on the basal lip annule, absence of longitudinal cuticular striations in anterior region, four lateral lines forming three equal bands which are areolated mainly at pharynx level, a robust stylet of 23 to 27 µm of which 45 to 53% is cone part, and with rounded to sometimes indented knobs, a secretory-excretory pore around level of pharyngo-intestinal junction, didelphic-amphidelphic reproductive system, vulva without distinct epiptygma, indistinct to empty spermatheca, tail usually truncated with 5 to 9 annuli, phasmids located at 7 to 17 annuli anterior to anus, and absence of males. Molecular phylogenies, in combination with species delimitation, supported the distinctiveness of <italic>Rotylenchus wimbii</italic> n. sp. and revealed some mislabeled <italic>Rotylenchus brevicaudatus</italic> sequences in GenBank.</p>
</abstract>

Rotylenchus wimbii n. sp. was found associated with finger millet in Kenya and is described based on light microscopy, scanning electron microscopy, and molecular information. Sequence analysis was performed on ITS, 18S, and D2-D3 of 28S of ribosomal DNA and COI of mitochondrial DNA. This new species is characterized by a moderate female body size of 0.6 to 0.8 mm, a continuous hemispherical lip region with four annuli, 3 to 4 irregular blocks on the basal lip annule, absence of longitudinal cuticular striations in anterior region, four lateral lines forming three equal bands which are areolated mainly at pharynx level, a robust stylet of 23 to 27 µm of which 45 to 53% is cone part, and with rounded to sometimes indented knobs, a secretory-excretory pore around level of pharyngo-intestinal junction, didelphic-amphidelphic reproductive system, vulva without distinct epiptygma, indistinct to empty spermatheca, tail usually truncated with 5 to 9 annuli, phasmids located at 7 to 17 annuli anterior to anus, and absence of males. Molecular phylogenies, in combination with species delimitation, supported the distinctiveness of Rotylenchus wimbii n. sp. and revealed some mislabeled Rotylenchus brevicaudatus sequences in GenBank.

<article-title xml:lang="en">
<italic>Rotylenchus wimbii</italic> n. sp. (Nematoda: Hoplolaimidae) associated with finger millet in Kenya

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


 n. sp. (Nematoda: Hoplolaimidae) associated with finger millet in Kenya

Rotylenchus wimbii n. sp. was found associated with finger millet in Kenya and is described based on light microscopy, scanning electron microscopy, and...

Rotylenchus wimbii n. sp. (Nematoda: Hoplolaimidae) associated with finger millet in Kenya

Publicado en línea: 01 ene 2021

Páginas: 1 - 14

DOI: https://doi.org/10.21307/jofnem-2021-016

Palabras clave
18S, 28S, ITS, Finger millet, Kenya, Morphometrics, New species, Phylogeny, Plant-parasitic nematodes, SEM, Systematics, Taxonomy

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Rotylenchus wimbii n. sp. (Nematoda: Hoplolaimidae) associated with finger millet in Kenya

Publicado en línea: 01 ene 2021

Páginas: 1 - 14

DOI: https://doi.org/10.21307/jofnem-2021-016

Palabras clave18S, 28S, ITS, Finger millet, Kenya, Morphometrics, New species, Phylogeny, Plant-parasitic nematodes, SEM, Systematics, Taxonomy

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
18S, 28S, ITS, Finger millet, Kenya, Morphometrics, New species, Phylogeny, Plant-parasitic nematodes, SEM, Systematics, Taxonomy