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Additional notes on the morphology and molecular data of the Kikuyu root-knot nematode, Meloidogyne kikuyensis (Nematoda: Meloidogynidae)

J. D. Eisenback
J. D. Eisenback
 y 
P. Vieira
P. Vieira
   | 12 ago 2020
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Publicado en línea: 12 ago 2020
Páginas: 1 - 13
DOI: https://doi.org/10.21307/jofnem-2020-067
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© 2020 J. D. Eisenback et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Light (LM) and scanning electron micrographs (SEM) of females of Meloidogyne kikuyensis De Grisse, 1961. A: LM of the anterior end showing the stylet and esophagus. B: SEM of an extracted stylet. C: LM of a whole specimen with attached gelatinous matrix secreted by the six rectal gland cells. D: SEM of female anterior end. E: SEM of the anterior end of a second-stage juvenile.
Light (LM) and scanning electron micrographs (SEM) of females of Meloidogyne kikuyensis De Grisse, 1961. A: LM of the anterior end showing the stylet and esophagus. B: SEM of an extracted stylet. C: LM of a whole specimen with attached gelatinous matrix secreted by the six rectal gland cells. D: SEM of female anterior end. E: SEM of the anterior end of a second-stage juvenile.

Figure 2:

Light micrographs (LM) of perineal patterns of Meloidogyne kikuyensis De Grisse, 1961. A-E: LM with polarizing rings in the condenser (Eisenback, 2010). F: LM utilizing typical brightfield microscopy.
Light micrographs (LM) of perineal patterns of Meloidogyne kikuyensis De Grisse, 1961. A-E: LM with polarizing rings in the condenser (Eisenback, 2010). F: LM utilizing typical brightfield microscopy.

Figure 3:

Light micrograph of a whole specimen of a male of Meloidogyne kikuyensis De Grisse, 1961.
Light micrograph of a whole specimen of a male of Meloidogyne kikuyensis De Grisse, 1961.

Figure 4:

Light (LM) and scanning electron micrographs (SEM) of males of Meloidogyne kikuyensis De Grisse, 1961. A: Anterior end. B: SEM of an excised stylet. C: SEM of an excised spicule.
Light (LM) and scanning electron micrographs (SEM) of males of Meloidogyne kikuyensis De Grisse, 1961. A: Anterior end. B: SEM of an excised stylet. C: SEM of an excised spicule.

Figure 5:

Scanning electron micrographs of the anterior end of males of Meloidogyne kikuyensis De Grisse, 1961. A: Face view showing a male with a distinct labial disk and six lips. B: Lateral view. C: Face view showing a male with just four lips. D: Medial view showing a male with six distinct lips.
Scanning electron micrographs of the anterior end of males of Meloidogyne kikuyensis De Grisse, 1961. A: Face view showing a male with a distinct labial disk and six lips. B: Lateral view. C: Face view showing a male with just four lips. D: Medial view showing a male with six distinct lips.

Figure 6:

Light micrograph of a second-stage juvenile of Meloidogyne kikuyensis De Grisse, 1961. A: Anterior end. B: Posterior end.
Light micrograph of a second-stage juvenile of Meloidogyne kikuyensis De Grisse, 1961. A: Anterior end. B: Posterior end.

Figure 7:

Light micrograph of second-stage juveniles of Meloidogyne kikuyensis De Grisse, 1961. A: Pre-infective migratory second-stage juvenile. B: Slightly swollen post-infective sedentary second-stage juvenile. C: Swollen post-infective second-stage juvenile.
Light micrograph of second-stage juveniles of Meloidogyne kikuyensis De Grisse, 1961. A: Pre-infective migratory second-stage juvenile. B: Slightly swollen post-infective sedentary second-stage juvenile. C: Swollen post-infective second-stage juvenile.

Figure 8:

Light micrographs comparing the second-stage juvenile of Meloidogyne kikuyensis De Grisse, 1961 with that of a typical root-knot species.
Light micrographs comparing the second-stage juvenile of Meloidogyne kikuyensis De Grisse, 1961 with that of a typical root-knot species.

Figure 9:

Light micrograph of eggs of Meloidogyne kikuyensis De Grisse, 1961. A: Single-cell stage. B: Two-cell stage with small, highly refractive polar cells (arrows). C: Four-cell stage. D: Eight-cell stage showing two small, highly refractive polar cells (arrows). E, F: Multi-cell stage of several eggs showing a ring of refractive cells (arrows) surrounding the developing embryo.
Light micrograph of eggs of Meloidogyne kikuyensis De Grisse, 1961. A: Single-cell stage. B: Two-cell stage with small, highly refractive polar cells (arrows). C: Four-cell stage. D: Eight-cell stage showing two small, highly refractive polar cells (arrows). E, F: Multi-cell stage of several eggs showing a ring of refractive cells (arrows) surrounding the developing embryo.

Figure 10:

Phylogenetic relationships based on the COII-16S sequences of Meloidogyne kikuyensis De Grisse, 1961 and other Meloidogyne species. The phylogenetic tree was deduced by maximum likelihood (ML) analysis with the HKY model and 1,000 bootstrap replicates (only values of bootstrap above 60% are shown). The COII-16S sequence of Radopholus similis was used as an outgroup.
Phylogenetic relationships based on the COII-16S sequences of Meloidogyne kikuyensis De Grisse, 1961 and other Meloidogyne species. The phylogenetic tree was deduced by maximum likelihood (ML) analysis with the HKY model and 1,000 bootstrap replicates (only values of bootstrap above 60% are shown). The COII-16S sequence of Radopholus similis was used as an outgroup.

Figure 11:

Phylogenetic relationships based on the ITS1 sequences of Meloidogyne kikuyensis De Grisse, 1961 and other Meloidogyne species. The phylogenetic tree was deduced by maximum likelihood (ML) analysis with the HKY model and 1,000 bootstrap replicates (only values of bootstrap above 60% are shown). The ITS1 sequence of Hirschmanniella mucronata was used as an outgroup.
Phylogenetic relationships based on the ITS1 sequences of Meloidogyne kikuyensis De Grisse, 1961 and other Meloidogyne species. The phylogenetic tree was deduced by maximum likelihood (ML) analysis with the HKY model and 1,000 bootstrap replicates (only values of bootstrap above 60% are shown). The ITS1 sequence of Hirschmanniella mucronata was used as an outgroup.
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