<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Two populations (CS19 and CS20) of entomopathogenic nematodes were isolated from the soils of vegetable fields from Bijnor district, india. Based on morphological, morphometrical, and molecular studies, the nematodes were identified as Steinernema surkhetense. This work represents the first report of this species in india. The infective juveniles (iJs) showed morphometrical and morphological differences, with the original description based on longer iJs size. The iJs of the indian isolates possess six ridges in their lateral field instead of eight reported in the original description. The analysis of iTs-rDNA sequences revealed nucleotide differences at 345, 608, and 920 positions in aligned data. No difference was observed in D2-D3 domain. The S. surkhetense Coi gene was studied for the first time as well as the molecular characterization of their Xenorhabdus symbiont using the sequences of recA and gyrB genes revealing Xenorhabdus stockiae as its symbiont. These data, together with the finding of X. stockiae, suggest that this bacterium is widespread among south Asian nematodes from the ‘‘carpocapsae’’ group. Virulence of both isolates was tested on Spodoptera litura. The strain Cs19 was capable to kill the larvae with 31.78 iJs at 72 hr, whereas Cs20 needed 67.7 iJs.</p>
</abstract>

Two populations (CS19 and CS20) of entomopathogenic nematodes were isolated from the soils of vegetable fields from Bijnor district, india. Based on morphological, morphometrical, and molecular studies, the nematodes were identified as Steinernema surkhetense. This work represents the first report of this species in india. The infective juveniles (iJs) showed morphometrical and morphological differences, with the original description based on longer iJs size. The iJs of the indian isolates possess six ridges in their lateral field instead of eight reported in the original description. The analysis of iTs-rDNA sequences revealed nucleotide differences at 345, 608, and 920 positions in aligned data. No difference was observed in D2-D3 domain. The S. surkhetense Coi gene was studied for the first time as well as the molecular characterization of their Xenorhabdus symbiont using the sequences of recA and gyrB genes revealing Xenorhabdus stockiae as its symbiont. These data, together with the finding of X. stockiae, suggest that this bacterium is widespread among south Asian nematodes from the ‘‘carpocapsae’’ group. Virulence of both isolates was tested on Spodoptera litura. The strain Cs19 was capable to kill the larvae with 31.78 iJs at 72 hr, whereas Cs20 needed 67.7 iJs.

First Report and Comparative Study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its Symbiont Bacteria from Subcontinental India

Department of Zoology, Chaudhary Charan Singh University

Centro de Estudios Botánicos y Agroforestales, Laboratorio de Protección Vegetal, Calle 79 conAv. 8 (Santa Rita)

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Two populations (CS19 and CS20) of entomopathogenic nematodes were isolated from the soils of vegetable fields from Bijnor district, india. Based on...

First Report and Comparative Study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its Symbiont Bacteria from Subcontinental India

Publicado en línea: 21 jul 2017

Páginas: 92 - 102

Recibido: 12 oct 2016

DOI: https://doi.org/10.21307/jofnem-2017-049

Palabras clave
D2-D3 domain, entomopathogenic nematode, iTs-rDNA, mt COI gene,

© 2017 Aashiq Hussain Bhat et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

First Report and Comparative Study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its Symbiont Bacteria from Subcontinental India

Publicado en línea: 21 jul 2017

Páginas: 92 - 102

Recibido: 12 oct 2016

DOI: https://doi.org/10.21307/jofnem-2017-049

Palabras claveD2-D3 domain, entomopathogenic nematode, iTs-rDNA, mt COI gene,

© 2017 Aashiq Hussain Bhat et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Palabras clave
D2-D3 domain, entomopathogenic nematode, iTs-rDNA, mt COI gene,