<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A solid-phase red cell adherence assay was used to demonstrate the specific inhibitory effect of seven species of <italic>Trimeresurus</italic> snake venom on the binding of HPA-1a- and HPA-1b-specific platelet antibodies. <italic>Trimeresurus</italic> venom did not inhibit the binding of HLA-, HPA-3a-, HPA-3b-, HPA-4a-, HPA-5a-, and HPA-5b-specific platelet antibodies. Venom from other genera of snakes, including representatives from <italic>Agkistrodon, Ancistrodon, Bitis, Bothrops, Bungarus, Causus, Crotalus, Dendroaspis, Ecis, Micrurus, Naja, Notechis, Ophiophagus, Pseudechis, Sepedon (Hemachatus)</italic>, and <italic>Vipera</italic>, all failed to specifically inhibit anti-HPA-1a and HPA-1b binding. These results may indicate that the component in <italic>Trimeresurus</italic> snake venom previously reported to bind to the platelet GPIIb-IIIa complex, inhibiting fibrinogen binding, binds close to the HPA-1a and HPA-1b epitopes. <bold><italic>Immunohematology</italic></bold> 1995;11:129–132.</p></abstract>

A solid-phase red cell adherence assay was used to demonstrate the specific inhibitory effect of seven species of Trimeresurus snake venom on the binding of HPA-1a- and HPA-1b-specific platelet antibodies. Trimeresurus venom did not inhibit the binding of HLA-, HPA-3a-, HPA-3b-, HPA-4a-, HPA-5a-, and HPA-5b-specific platelet antibodies. Venom from other genera of snakes, including representatives from Agkistrodon, Ancistrodon, Bitis, Bothrops, Bungarus, Causus, Crotalus, Dendroaspis, Ecis, Micrurus, Naja, Notechis, Ophiophagus, Pseudechis, Sepedon (Hemachatus), and Vipera, all failed to specifically inhibit anti-HPA-1a and HPA-1b binding. These results may indicate that the component in Trimeresurus snake venom previously reported to bind to the platelet GPIIb-IIIa complex, inhibiting fibrinogen binding, binds close to the HPA-1a and HPA-1b epitopes. Immunohematology 1995;11:129–132.

<italic>Trimeresurus</italic> venom inhibition of anti-HPA-1a and anti-HPA-1b antibody binding to human platelets

Immunohematology

 venom inhibition of anti-HPA-1a and anti-HPA-1b antibody binding to human platelets

A solid-phase red cell adherence assay was used to demonstrate the specific inhibitory effect of seven species of Trimeresurus snake venom on the binding of...

Trimeresurus venom inhibition of anti-HPA-1a and anti-HPA-1b antibody binding to human platelets

Publicado en línea: 17 nov 2020

Páginas: 129 - 132

DOI: https://doi.org/10.21307/immunohematology-2019-803

© 1995 S.J. Wlodar et al., published by Sciendo