<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A chimeric individual possesses two or more genetically distinct ceil populations. Although the chimerism may not be evident in all gene systems, various loci display greater numbers of alleles than genetically “normal” individ uals. The proposit’a was referred for further laboratory investigation due to a mixed-field ABO blood group reaction following routine antenatal testing. Various molecular (HLA class II, ABO genotyping, and 10 short tandem repeat [STR] microsatellites) and serologic (HLA class I and red cell blood groups) typing techniques were employed to investigate a number of polymorphic loci located on different chromosomes. Chimerism was identified in 8 out of the 1.4 chromosomes tested: chromosome 1 (Duffy), 6 (HLA class I and Iï), 9 (ABO), 11 (<italic>HUMTH01</italic>), 12 (<italic>HUMPLA2A1</italic>), 15 (<italic>HUMFES/FPS</italic>). 18 (Kidd) and 21 (<italic>D21S11</italic>). The proposita was determined to be a probable dispermic chimera, based on the results of the serology and molecular studies.</p></abstract>

A chimeric individual possesses two or more genetically distinct ceil populations. Although the chimerism may not be evident in all gene systems, various loci display greater numbers of alleles than genetically “normal” individ uals. The proposit’a was referred for further laboratory investigation due to a mixed-field ABO blood group reaction following routine antenatal testing. Various molecular (HLA class II, ABO genotyping, and 10 short tandem repeat [STR] microsatellites) and serologic (HLA class I and red cell blood groups) typing techniques were employed to investigate a number of polymorphic loci located on different chromosomes. Chimerism was identified in 8 out of the 1.4 chromosomes tested: chromosome 1 (Duffy), 6 (HLA class I and Iï), 9 (ABO), 11 (HUMTH01), 12 (HUMPLA2A1), 15 (HUMFES/FPS). 18 (Kidd) and 21 (D21S11). The proposita was determined to be a probable dispermic chimera, based on the results of the serology and molecular studies.

Serologic and molecular investigations of a chimera

Immunohematology

{"article-title":"Serologic and molecular investigations of a chimera"}

A chimeric individual possesses two or more genetically distinct ceil populations. Although the chimerism may not be evident in all gene systems, various loci...

Serologic and molecular investigations of a chimera

Publicado en línea: 26 oct 2020

Páginas: 100 - 104

DOI: https://doi.org/10.21307/immunohematology-2019-626

Palabras clave
chimela, disperrnic, serolobq, HLA, DNA analvsis

© 1999 N. A. MIFSUD, et al., published by Sciendo

Serologic and molecular investigations of a chimera

Publicado en línea: 26 oct 2020

Páginas: 100 - 104

DOI: https://doi.org/10.21307/immunohematology-2019-626

Palabras clavechimela, disperrnic, serolobq, HLA, DNA analvsis

© 1999 N. A. MIFSUD, et al., published by Sciendo

Palabras clave
chimela, disperrnic, serolobq, HLA, DNA analvsis