<abstract xmlns="http://www.w3.org/1999/xhtml">Serologic ABO blood typing is routinely performed using anti-A and anti-B sera to distinguish four phenotypes (A, B, AB, and O). Restriction fragment length polymorphisms (RFLPs) and DNA sequence studies offer the possibility of direct ABO genotyping. We used polymerase chain reaction-RFLP analysis to determine the frequency of <italic>O1</italic> and <italic>O2</italic> alleles in 82 unrelated blood donors in São Paulo, Brazil, known to be group O. Genomic DNA was extracted from blood leukocytes by a modified salting-out method. Different genotypes <italic>(O1O1, O1O2, O2O2)</italic> were identified after digestion with restriction enzymes <italic>Kpn</italic>I, <italic>Hpa</italic>II, and <italic>Alu</italic>I, followed by agarose gel electrophoresis. Of 82 samples analyzed, 74 were <italic>O1O1</italic>, 7 were <italic>O1O2</italic>, and 1 was <italic>O2O2</italic>. These results showed the frequency of <italic>O1O1, O1O2</italic>, and <italic>O2O2</italic> genotypes to be 90.24 percent, 8.53 percent, and 1.22 percent, respectively, in blood donors in São Paulo, Brazil. <bold><italic>Immunohematology</italic> 2001;17:111–116</bold>.</abstract>

Serologic ABO blood typing is routinely performed using anti-A and anti-B sera to distinguish four phenotypes (A, B, AB, and O). Restriction fragment length polymorphisms (RFLPs) and DNA sequence studies offer the possibility of direct ABO genotyping. We used polymerase chain reaction-RFLP analysis to determine the frequency of O1 and O2 alleles in 82 unrelated blood donors in São Paulo, Brazil, known to be group O. Genomic DNA was extracted from blood leukocytes by a modified salting-out method. Different genotypes (O1O1, O1O2, O2O2) were identified after digestion with restriction enzymes KpnI, HpaII, and AluI, followed by agarose gel electrophoresis. Of 82 samples analyzed, 74 were O1O1, 7 were O1O2, and 1 was O2O2. These results showed the frequency of O1O1, O1O2, and O2O2 genotypes to be 90.24 percent, 8.53 percent, and 1.22 percent, respectively, in blood donors in São Paulo, Brazil. Immunohematology 2001;17:111–116.

Easy method for determining the frequency of <italic>O1</italic> and <italic>O2</italic> alleles in Brazilian blood donors by PCR-RFLP analysis

Immunohematology

Easy method for determining the frequency of  and  alleles in Brazilian blood donors by PCR-RFLP analysis

Serologic ABO blood typing is routinely performed using anti-A and anti-B sera to distinguish four phenotypes (A, B, AB, and O). Restriction fragment length...

Easy method for determining the frequency of O¹ and O² alleles in Brazilian blood donors by PCR-RFLP analysis

Publicado en línea: 14 oct 2020

Páginas: 111 - 116

DOI: https://doi.org/10.21307/immunohematology-2019-563

Palabras clave
ABO blood group, genotypes, allele, allele, blood donors, PCR, RFLP, blood group frequencies

© 2019 A.C. Batissoco et al., published by Sciendo

Easy method for determining the frequency of O1 and O2 alleles in Brazilian blood donors by PCR-RFLP analysis