<abstract xmlns="http://www.w3.org/1999/xhtml"><p>While studying the serologic characteristics of certain monoclonal blood group antibodies, we observed a hybridoma clone (5A- 11E10) with anti-N–like serologic specificity that was dependent on the presence of the bicarbonate anion. The diluted cell culture supernatant preferentially agglutinated M–N+ RBCs by immediate spin. This supernatant also agglutinated M–N+ RBCs that had been treated with trypsin or sialidase (to remove N-reactivity), suggesting anti-’N’ activity. Anti-’N’ specificity was confirmed by the supernatant’s non-reactivity with N+ RBCs treated with papain (to remove ‘N’ reactivity) or with (‘N’-negative) M+N–U– RBCs. The requirement for bicarbonate in the MoAb’s formulation was not a function of pH. Both sodium and ammonium bicarbonate supported agglutination, but neither sulfate nor carbonate was effective.</p></abstract>

While studying the serologic characteristics of certain monoclonal blood group antibodies, we observed a hybridoma clone (5A- 11E10) with anti-N–like serologic specificity that was dependent on the presence of the bicarbonate anion. The diluted cell culture supernatant preferentially agglutinated M–N+ RBCs by immediate spin. This supernatant also agglutinated M–N+ RBCs that had been treated with trypsin or sialidase (to remove N-reactivity), suggesting anti-’N’ activity. Anti-’N’ specificity was confirmed by the supernatant’s non-reactivity with N+ RBCs treated with papain (to remove ‘N’ reactivity) or with (‘N’-negative) M+N–U– RBCs. The requirement for bicarbonate in the MoAb’s formulation was not a function of pH. Both sodium and ammonium bicarbonate supported agglutination, but neither sulfate nor carbonate was effective.

A bicarbonate anion-dependent anti-’N’ MoAb

Immunohematology

{"article-title":"A bicarbonate anion-dependent anti-\u2019N\u2019 MoAb"}

While studying the serologic characteristics of certain monoclonal blood group antibodies, we observed a hybridoma clone (5A- 11E10) with anti-N–like serologic...

A bicarbonate anion-dependent anti-’N’ MoAb

Publicado en línea: 02 may 2020

Páginas: 59 - 62

DOI: https://doi.org/10.21307/immunohematology-2019-422

Palabras clave
anti-N, anti-’N’, monoclonoal antibody, bicarbonate-dependent antibody

© 2004 Y.S. Iyer et al., published by Sciendo

A bicarbonate anion-dependent anti-’N’ MoAb

Publicado en línea: 02 may 2020

Páginas: 59 - 62

DOI: https://doi.org/10.21307/immunohematology-2019-422

Palabras claveanti-N, anti-’N’, monoclonoal antibody, bicarbonate-dependent antibody

© 2004 Y.S. Iyer et al., published by Sciendo

Palabras clave
anti-N, anti-’N’, monoclonoal antibody, bicarbonate-dependent antibody