<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Flow cytometric methods commonly used to identify reticulocytes are of limited usefulness in malarious areas, since RNA staining also detects plasmodia. An important antigen expressed on reticulocytes is Duffy antigen receptor for chemokines (DARC, also known as Fy), the receptor for <italic>Plasmodium vivax</italic>. An early marker for reticulocytes is CD71 (transferrin receptor). We have been interested in CD71 as an alternative marker for reticulocytes in the context of Fy expression. Flow cytometry was used to determine the expression of Fy on CD71-positive and -negative reticulocytes and to correlate serology and genotype. A reduction of 13 percent was seen in Fy6 expression between CD71-positive reticulocytes and RNA-positive reticulocytes. CD71 disappears early during reticulocyte maturation, while Fy6 expression is relatively preserved. CD71 is an alternative to staining for RNA for reticulocyte assays relating to Fy6 expression. <italic>
<bold>Immunohematology</bold>
</italic> <bold>2005;21:15–20.</bold>
</p>
</abstract>

Flow cytometric methods commonly used to identify reticulocytes are of limited usefulness in malarious areas, since RNA staining also detects plasmodia. An important antigen expressed on reticulocytes is Duffy antigen receptor for chemokines (DARC, also known as Fy), the receptor for Plasmodium vivax. An early marker for reticulocytes is CD71 (transferrin receptor). We have been interested in CD71 as an alternative marker for reticulocytes in the context of Fy expression. Flow cytometry was used to determine the expression of Fy on CD71-positive and -negative reticulocytes and to correlate serology and genotype. A reduction of 13 percent was seen in Fy6 expression between CD71-positive reticulocytes and RNA-positive reticulocytes. CD71 disappears early during reticulocyte maturation, while Fy6 expression is relatively preserved. CD71 is an alternative to staining for RNA for reticulocyte assays relating to Fy6 expression. 
Immunohematology
 2005;21:15–20.

Expression of Duffy antigen receptor for chemokines during reticulocyte maturation: using a CD71 flow cytometric technique to identify reticulocytes

Immunohematology

Flow cytometric methods commonly used to identify reticulocytes are of limited usefulness in malarious areas, since RNA staining also detects plasmodia. An...

Expression of Duffy antigen receptor for chemokines during reticulocyte maturation: using a CD71 flow cytometric technique to identify reticulocytes

Publicado en línea: 20 abr 2020

Páginas: 15 - 20

DOI: https://doi.org/10.21307/immunohematology-2019-387

Palabras clave
DARC, Fy, reticulocyte, CD71, transferrin receptor, ORF, flow cytometry

© 2005 I.J Woolley, et al., published by Sciendo

Expression of Duffy antigen receptor for chemokines during reticulocyte maturation: using a CD71 flow cytometric technique to identify reticulocytes

Publicado en línea: 20 abr 2020

Páginas: 15 - 20

DOI: https://doi.org/10.21307/immunohematology-2019-387

Palabras claveDARC, Fy, reticulocyte, CD71, transferrin receptor, ORF, flow cytometry

© 2005 I.J Woolley, et al., published by Sciendo

Palabras clave
DARC, Fy, reticulocyte, CD71, transferrin receptor, ORF, flow cytometry