<abstract xmlns="http://www.w3.org/1999/xhtml">

Human platelet alloantigens (HPA) are important in neonatal alloimmune thrombocytopenia (NAIT), posttransfusion purpura (PTP), platelet transfusion refractoriness, passive alloimmune thrombocytopenia, and transplantation-associated alloimmune thrombocytopenia. Thus, <italic>HPA</italic> genotyping is essential in diagnosis and treatment. We analyzed <italic>HPA-1</italic> to <italic>6</italic> and <italic>Gov</italic> alleles, using PCR with sequence specific primers (PCR-SSP) in 500 Thai blood donors who had been HLA class I antigen typed. <italic>HPA-4a</italic> was present in all samples. <italic>HPA-1b, -2b, -5b</italic>, and -<italic>6b</italic> were rare, and <italic>HPA-4b</italic> was not found. <italic>HPA-3a</italic> and <italic>-3b</italic> showed frequencies of 56.0 percent and 44.0 percent, respectively. <italic>Gova
</italic> and <italic>Govb
</italic> showed frequencies of 49.1 percent and 50.9 percent, respectively. The prevalence rates of <italic>HPA-1</italic> to <italic>6</italic> gene frequencies (GFs) were consistent with those of other Asian populations rather than those of Caucasians. We also report on the GFs of <italic>Gova
</italic> and <italic>Govb
</italic>, which also are comparable to those of Asian populations. Our results could establish a useful HPA- and HLA-matched plateletpheresis donor file and provide an improvement of platelet alloantibody detection in alloimmune thrombocytopenic patients, and, therefore, a more effective platelet transfusion program. <italic>
<bold>Immunohematology</bold>
</italic> <bold>2005;21:5–9.</bold>

</abstract>

Human platelet alloantigens (HPA) are important in neonatal alloimmune thrombocytopenia (NAIT), posttransfusion purpura (PTP), platelet transfusion refractoriness, passive alloimmune thrombocytopenia, and transplantation-associated alloimmune thrombocytopenia. Thus, HPA genotyping is essential in diagnosis and treatment. We analyzed HPA-1 to 6 and Gov alleles, using PCR with sequence specific primers (PCR-SSP) in 500 Thai blood donors who had been HLA class I antigen typed. HPA-4a was present in all samples. HPA-1b, -2b, -5b, and -6b were rare, and HPA-4b was not found. HPA-3a and -3b showed frequencies of 56.0 percent and 44.0 percent, respectively. Gova
 and Govb
 showed frequencies of 49.1 percent and 50.9 percent, respectively. The prevalence rates of HPA-1 to 6 gene frequencies (GFs) were consistent with those of other Asian populations rather than those of Caucasians. We also report on the GFs of Gova
 and Govb
, which also are comparable to those of Asian populations. Our results could establish a useful HPA- and HLA-matched plateletpheresis donor file and provide an improvement of platelet alloantibody detection in alloimmune thrombocytopenic patients, and, therefore, a more effective platelet transfusion program. 
Immunohematology
 2005;21:5–9.

Gene frequencies of the HPA-1 to 6 and Gov human platelet antigens in Thai blood donors

Department of Clinical Microscopy, Mahidol University

Immunohematology

Human platelet alloantigens (HPA) are important in neonatal alloimmune thrombocytopenia (NAIT), posttransfusion purpura (PTP), platelet transfusion...

Gene frequencies of the HPA-1 to 6 and Gov human platelet antigens in Thai blood donors

Publicado en línea: 20 abr 2020

Páginas: 5 - 9

DOI: https://doi.org/10.21307/immunohematology-2019-385

Palabras clave
human platelet antigen (HPA), Gov, PCR-SSP, Thais, gene frequencies

© 2005 P. Kupatawintu, et al., published by Sciendo

Gene frequencies of the HPA-1 to 6 and Gov human platelet antigens in Thai blood donors

Publicado en línea: 20 abr 2020

Páginas: 5 - 9

DOI: https://doi.org/10.21307/immunohematology-2019-385

Palabras clavehuman platelet antigen (HPA), Gov, PCR-SSP, Thais, gene frequencies

© 2005 P. Kupatawintu, et al., published by Sciendo

Palabras clave
human platelet antigen (HPA), Gov, PCR-SSP, Thais, gene frequencies