<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Correct donor D typing is critical to prevent recipient alloimmunization. No method can detect all variants, and the immunogenicity of many variants is unknown. Routine ABO and D serologic typings are performed in our laboratory by automated microplate testing. Until 2011, routine confirmation of D– status of first-time donors was performed by the manual tube indirect antiglobulin test (IAT); this was replaced by automated solid-phase testing including weak D testing by IAT. Selected donors are investigated by other methods. We describe four weak D type 67 <italic>(RHD*01W.67)</italic> donors whose samples tested as D– by automated microplate and manual methods but were later determined to be D+ by automated solid-phase and <italic>RHD</italic> gene analysis. Solid-phase serologic and molecular typing results of all four donors were identical. It was identified that the donors are of English-Irish descent; two are brothers and the others are cousins. Transfusion of blood from one of these donors likely resulted in alloimmunization to D in one of three recipients tested since no other documented exposures were identified. Lookback studies determined that two other D– recipients were not alloimmunized. <italic><bold>Immunohematology</bold></italic><bold>2015;31:159–162.</bold></p></abstract>

Correct donor D typing is critical to prevent recipient alloimmunization. No method can detect all variants, and the immunogenicity of many variants is unknown. Routine ABO and D serologic typings are performed in our laboratory by automated microplate testing. Until 2011, routine confirmation of D– status of first-time donors was performed by the manual tube indirect antiglobulin test (IAT); this was replaced by automated solid-phase testing including weak D testing by IAT. Selected donors are investigated by other methods. We describe four weak D type 67 (RHD*01W.67) donors whose samples tested as D– by automated microplate and manual methods but were later determined to be D+ by automated solid-phase and RHD gene analysis. Solid-phase serologic and molecular typing results of all four donors were identical. It was identified that the donors are of English-Irish descent; two are brothers and the others are cousins. Transfusion of blood from one of these donors likely resulted in alloimmunization to D in one of three recipients tested since no other documented exposures were identified. Lookback studies determined that two other D– recipients were not alloimmunized. Immunohematology2015;31:159–162.

Weak D type 67 in four related Canadian blood donors

Immunohematology

{"article-title":"Weak D type 67 in four related Canadian blood donors"}

Correct donor D typing is critical to prevent recipient alloimmunization. No method can detect all variants, and the immunogenicity of many variants is...

Weak D type 67 in four related Canadian blood donors

Article Category: Case Report

Publicado en línea: 26 oct 2019

Páginas: 159 - 162

DOI: https://doi.org/10.21307/immunohematology-2019-086

Palabras clave
variant, alloimmunization, weak D

© 2015 P. Berardi et al., published by Sciendo

Weak D type 67 in four related Canadian blood donors

Article Category: Case Report

Publicado en línea: 26 oct 2019

Páginas: 159 - 162

DOI: https://doi.org/10.21307/immunohematology-2019-086

Palabras clavevariant, alloimmunization, weak D

© 2015 P. Berardi et al., published by Sciendo

Palabras clave
variant, alloimmunization, weak D