Susceptibility of spirochetes Borreliaburgdorferisensulato to antibiotics in vitro

Empiric therapy has been applied in the treatment of Lyme disease. This therapy is selected following the sensitivity analysis of the proposed drug in all species of bacteria which can cause a similar type of infection and on the basis of the clinical efficacy of antibiotic treatment. Established schemes based on data collected from many centers in the world, including type of antibiotic, dose and duration of his administration, and the stage and form of Lyme disease have been created. Number of in vitromethods of spirochetes susceptibility to antibiotics has been also developed. Unfortunately, the lack of standardization often makes it impossible to compare the results of MIC and MBC. Furthermore, little is known about the interactions of the various antimicrobial substances and spirochetes. There is a need for testing of clinical strains isolated from patients after treatment, which would explain the problems associated with “refractory” cases of Lyme disease. The paper presents the research on the antibiotic-spirochete interactions observed in vitro.

1. Introduction. 2. In vitro culture and growth of Borreliaburgdorferisensulato. 3. In vitro susceptibility of Borreliaburgdorferisensustricto strains to antimicrobial agents. 4. Summary