<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Cross-species amplification of six microsatellite markers from European beech (Fagus sylvatica Linn) and nine markers from Japanese beech (Fagus crenata Blume) was tested in American beech (Fagus grandifolia Ehrh.). Three microsatellites from each species were successfully adapted for use in American beech and were found to be highly polymorphic, with 4-22 alleles at each locus and an expected heterozygosity value of 0.291 to 0.913. Twenty-five trees (including two clonal clusters) from a mature stand were sampled and genotyped to compute population statistics. No linkage disequilibrium between pairs of loci was detected, and the marker loci indicated that the population is at Hardy- Weinberg equilibrium. The markers were also used to genotype two full-sibling families consisting of a combined total of 99 individuals and were found to contain sufficient genetic information to assign paternity using a maximum likelihood method.</p></abstract>

Cross-species amplification of six microsatellite markers from European beech (Fagus sylvatica Linn) and nine markers from Japanese beech (Fagus crenata Blume) was tested in American beech (Fagus grandifolia Ehrh.). Three microsatellites from each species were successfully adapted for use in American beech and were found to be highly polymorphic, with 4-22 alleles at each locus and an expected heterozygosity value of 0.291 to 0.913. Twenty-five trees (including two clonal clusters) from a mature stand were sampled and genotyped to compute population statistics. No linkage disequilibrium between pairs of loci was detected, and the marker loci indicated that the population is at Hardy- Weinberg equilibrium. The markers were also used to genotype two full-sibling families consisting of a combined total of 99 individuals and were found to contain sufficient genetic information to assign paternity using a maximum likelihood method.

Use of Microsatellite Markers in an American Beech (Fagus grandifolia) Population and Paternity Testing

Silvae Genetica

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Cross-species amplification of six microsatellite markers from European beech (Fagus sylvatica Linn) and nine markers from Japanese beech (Fagus crenata Blume)...

Use of Microsatellite Markers in an American Beech (Fagus grandifolia) Population and Paternity Testing

Publicado en línea: 20 oct 2017

Páginas: 62 - 68

Recibido: 22 oct 2008

DOI: https://doi.org/10.1515/sg-2010-0008

Palabras clave
beech bark disease, microsatellite markers, paternity testing, population genetics

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Use of Microsatellite Markers in an American Beech (Fagus grandifolia) Population and Paternity Testing

Publicado en línea: 20 oct 2017

Páginas: 62 - 68

Recibido: 22 oct 2008

DOI: https://doi.org/10.1515/sg-2010-0008

Palabras clavebeech bark disease, microsatellite markers, paternity testing, population genetics

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
beech bark disease, microsatellite markers, paternity testing, population genetics