Name of primer | Sequence (5˘-3˘) | Annealing temp (°C) | Product size (bp) | DMSO % | MgCl2 mM |
---|---|---|---|---|---|
FOXC2-1F = Primer pairs from |
TCTGGCTCTCTCGCGCTCT | 58 | 476 | 6 | 1.5 |
FKHL14-2R | AGTAACTGCCCTTGCCGG | ||||
FOXC2-2F = Primer pairs from |
ACCGCTTCCCCTTCTACCGG | 60 | 519 | 10 | 1.5 |
FOXC2-2R | TCATGATGTTCTCCACGCTGAA | ||||
FKHL14-4F = Primer pairs from |
GAAGGTGGTGATCAAGAGCG | 60 | 496 | 6 | 1.5 |
FOXC2-3R | GAGGTTGAGAGCGCTCAGGG | ||||
FOXC2-4F = Primer pairs from |
CTGGACGAGGCCCTCTCGGAC | 61 | 464 | 10 | 1.5 |
FOXC2-4R | GGAGGTCCCGGGACACGTCA | ||||
FOX_5P_1F = Primer pairs from |
GCCGACGGATTCCTGCGCTC | 61 | 378 | 10 | 1.5 |
FOX_5P_1R | CCGCTCCTCGCTGGCTCCA | ||||
FOX_5P_2F = Primer pairs from |
CCGATTCGCTGGGGGCTTGGAG | 61 | 607 | 6 | 1.5 |
FOX_5P_2R | GCGGGCTGGTGGTGGTGGTAGG | ||||
FOX_3P_1F = Primer pairs from |
CAACGTGCGGGAGATGTTCAAC | 61 | 464 | 10 | 1.5 |
FOX_3P_1R | CACAGCACAGCCGTCCTGGTAG | ||||
FOX_3P_2F = Primer pairs from |
TACTGACGTGTCCCGGGACC | 61 | 468 | 6 | 1.5 |
FOX_3P_2R | CCACACATTTGTACAGCACGGTTG |
Patients | |||||
---|---|---|---|---|---|
Gender | M | F | M | F | F |
Age (years) | 74 | 39 | 14 | 9 | 6 |
Lymphoedema | Yes | Yes | Yes | No | No |
Lower limbs | Yes = whole lower limbs |
Yes = whole lower limbs |
Yes = calves only |
No | No |
Genital | Yes | No | No | No | No |
Distichiasis | No | No | No | No | No |
Onset (years) | 11 | 9 | 13 | / | / |
Varicose veins | Yes | Yes | No | No | No |
Ptosis | No | No | No | No | No |
Cleft palate | No | No | No | No | No |
Congenital heart disease | No | No | No | No | No |
c.867insA | c.867insA | c.867insA | No | c.867insA | |
Cellulitis | Yes | Yes | No | No | No |
Yellow nails | No | No | No | No | No |