Expression of LOC285758, a potential long non-coding biomarker, is methylation-dependent and correlates with glioma malignancy grade
Categoría del artículo: Research Article
Publicado en línea: 14 ene 2017
Páginas: 331 - 341
Recibido: 24 oct 2016
Aceptado: 22 nov 2016
DOI: https://doi.org/10.1515/raon-2017-0004
Palabras clave
© 2017 Alenka Matjasic, Mara Popovic, Bostjan Matos and Damjan Glavac
This work is licensed under the Creative Commons Attribution 4.0 International License.
Background
Identifying the early genetic drivers can help diagnose glioma tumours in their early stages, before becoming malignant. However, there is emerging evidence that disturbance of epigenetic mechanisms also contributes to cell’s malignant transformation and cancer progression. Long non-coding RNAs are one of key epigenetic modulators of signalling pathways, since gene expression regulation is one of their canonical mechanisms. The aim of our study was to search new gliomagenesis-specific candidate lncRNAs involved in epigenetic regulation.
Patients and methods
We used a microarray approach to detect expression profiles of epigenetically involved lncRNAs on a set of 12 glioma samples, and selected
Results
In all glioma subtypes levels of LOC285758 were significantly higher in comparison to normal brain reference RNA, and expression was inversely associated with promoter methylation. Expression substantially differs between astrocytoma and oligodendroglioma, and is elevated in higher WHO grades, which also showed loss of methylation.
Conclusions
Our study revealed that lncRNA