<abstract xmlns="http://www.w3.org/1999/xhtml">The paper describes the visualization of the cells (ESEs) and mucilage (ECMSN) in an embryogenic tissue via magnetic resonance imaging (MRI) relaxometry measurement combined with the subsequent multi-parametric segmentation. The computed relaxometry maps <italic>T</italic>1 and <italic>T</italic>2 show a thin layer (transition layer) between the culture medium and the embryogenic tissue. The ESEs, mucilage, and transition layer differ in their relaxation times <italic>T</italic>1 and <italic>T</italic>2; thus, these times can be used to characterize the individual parts within the embryogenic tissue. The observed mean values of the relaxation times <italic>T</italic>1 and <italic>T</italic>2 of the ESEs, mucilage, and transition layer are as follows: 1469 ± 324 and 53 ± 10 ms, 1784 ± 124 and 74 ± 8 ms, 929 ± 164 and 32 ± 4.7 ms, respectively. The multi-parametric segmentation exploiting the <italic>T</italic>1 and <italic>T</italic>2 relaxation times as a classifier shows the distribution of the ESEs and mucilage within the embryogenic tissue. The discussed <italic>T</italic>1 and <italic>T</italic>2 indicators can be utilized to characterize both the growth-related changes in an embryogenic tissue and the effect of biotic/abiotic stresses, thus potentially becoming a distinctive indicator of the state of any examined embryogenic tissue.</abstract>

The paper describes the visualization of the cells (ESEs) and mucilage (ECMSN) in an embryogenic tissue via magnetic resonance imaging (MRI) relaxometry measurement combined with the subsequent multi-parametric segmentation. The computed relaxometry maps T1 and T2 show a thin layer (transition layer) between the culture medium and the embryogenic tissue. The ESEs, mucilage, and transition layer differ in their relaxation times T1 and T2; thus, these times can be used to characterize the individual parts within the embryogenic tissue. The observed mean values of the relaxation times T1 and T2 of the ESEs, mucilage, and transition layer are as follows: 1469 ± 324 and 53 ± 10 ms, 1784 ± 124 and 74 ± 8 ms, 929 ± 164 and 32 ± 4.7 ms, respectively. The multi-parametric segmentation exploiting the T1 and T2 relaxation times as a classifier shows the distribution of the ESEs and mucilage within the embryogenic tissue. The discussed T1 and T2 indicators can be utilized to characterize both the growth-related changes in an embryogenic tissue and the effect of biotic/abiotic stresses, thus potentially becoming a distinctive indicator of the state of any examined embryogenic tissue.

Characterization of the Embryogenic Tissue of the Norway Spruce Including a Transition Layer between the Tissue and the Culture Medium by Magnetic Resonance Imaging

Institute of Scientific Instruments of the CAS, Kralovopolska, 147, 612 64, Brno,

Brno University of Technology, Faculty of Electrotechnical Engineering and Communication, Department of Theoretical and Experimental Electrical Engineering, Technická, 3082/12, 616 00, Brno,

Mendel University in Brno, Faculty of Agronomy, Department of Plant Biology, Zemedelska, 1, 613 00, Brno,

Measurement Science Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The paper describes the visualization of the cells (ESEs) and mucilage (ECMSN) in an embryogenic tissue via magnetic resonance imaging (MRI) relaxometry...

Characterization of the Embryogenic Tissue of the Norway Spruce Including a Transition Layer between the Tissue and the Culture Medium by Magnetic Resonance Imaging

Publicado en línea: 23 feb 2017

Páginas: 19 - 26

Recibido: 23 ago 2016

Aceptado: 25 ene 2017

DOI: https://doi.org/10.1515/msr-2017-0003

Palabras clave
Norway spruce, ESEs, mucilage, transition layer, MRI relaxometry

© 2017 R. Kořínek et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Characterization of the Embryogenic Tissue of the Norway Spruce Including a Transition Layer between the Tissue and the Culture Medium by Magnetic Resonance Imaging

Publicado en línea: 23 feb 2017

Páginas: 19 - 26

Recibido: 23 ago 2016

Aceptado: 25 ene 2017

DOI: https://doi.org/10.1515/msr-2017-0003

Palabras claveNorway spruce, ESEs, mucilage, transition layer, MRI relaxometry

© 2017 R. Kořínek et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
Norway spruce, ESEs, mucilage, transition layer, MRI relaxometry