<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The prevention and control of <italic>Actinobacillus pleuropneumoniae</italic> in commercial production settings is based on serological monitoring. Enzyme-linked immunosorbent assays (ELISAs) have been developed to detect specific antibodies against a variety of <italic>A. pleuropneumoniae</italic> antigens, including long-chain lipopolysaccharides (LPS) and the ApxIV toxin, a repeats-in-toxin (RTX) exotoxin unique to <italic>A. pleuropneumoniae</italic> and produced by all serovars. The objective of this study was to describe ApxIV antibody responses in serum and oral fluid of pigs.<bold>Material and Methods:</bold> Four groups of pigs (six pigs per group) were inoculated with <italic>A. pleuropneumoniae</italic> serovars 1, 5, 7, or 12. Weekly serum samples and daily oral fluid samples were collected from individual pigs for 56 days post inoculation (DPI) and tested by LPS and ApxIV ELISAs. The ApxIV ELISA was run in three formats to detect immunlgobulins M, G, and A (IgM, IgG and IgA) while the LPS ELISA detected only IgG.<bold>Results:</bold> All pigs inoculated with <italic>A. pleuropneumoniae</italic> serovars 1 and 7 were LPS ELISA serum antibody positive from DPI 14 to 56. A transient and weak LPS ELISA antibody response was observed in pigs inoculated with serovar 5 and a single antibody positive pig was observed in serovar 12 at ≥35 DPI. Notably, ApxIV serum and oral fluid antibody responses in pig inoculated with serovars 1 and 7 reflected the patterns observed for LPS antibody, albeit with a 14 to 21 day delay.<bold>Conclusion:</bold> This work suggests that ELISAs based on ApxIV antibody detection in oral fluid samples could be effective in population monitoring for <italic>A. pleuropneumoniae</italic>.</abstract>

Introduction: The prevention and control of Actinobacillus pleuropneumoniae in commercial production settings is based on serological monitoring. Enzyme-linked immunosorbent assays (ELISAs) have been developed to detect specific antibodies against a variety of A. pleuropneumoniae antigens, including long-chain lipopolysaccharides (LPS) and the ApxIV toxin, a repeats-in-toxin (RTX) exotoxin unique to A. pleuropneumoniae and produced by all serovars. The objective of this study was to describe ApxIV antibody responses in serum and oral fluid of pigs.Material and Methods: Four groups of pigs (six pigs per group) were inoculated with A. pleuropneumoniae serovars 1, 5, 7, or 12. Weekly serum samples and daily oral fluid samples were collected from individual pigs for 56 days post inoculation (DPI) and tested by LPS and ApxIV ELISAs. The ApxIV ELISA was run in three formats to detect immunlgobulins M, G, and A (IgM, IgG and IgA) while the LPS ELISA detected only IgG.Results: All pigs inoculated with A. pleuropneumoniae serovars 1 and 7 were LPS ELISA serum antibody positive from DPI 14 to 56. A transient and weak LPS ELISA antibody response was observed in pigs inoculated with serovar 5 and a single antibody positive pig was observed in serovar 12 at ≥35 DPI. Notably, ApxIV serum and oral fluid antibody responses in pig inoculated with serovars 1 and 7 reflected the patterns observed for LPS antibody, albeit with a 14 to 21 day delay.Conclusion: This work suggests that ELISAs based on ApxIV antibody detection in oral fluid samples could be effective in population monitoring for A. pleuropneumoniae.

Detection of <italic>Actinobacillus pleuropneumoniae</italic> ApxIV toxin antibody in serum and oral fluid specimens from pigs inoculated under experimental conditions

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detection of  ApxIV toxin antibody in serum and oral fluid specimens from pigs inoculated under experimental conditions

Introduction: The prevention and control of Actinobacillus pleuropneumoniae in commercial production settings is based on serological monitoring. Enzyme-linked...

Detection of Actinobacillus pleuropneumoniae ApxIV toxin antibody in serum and oral fluid specimens from pigs inoculated under experimental conditions

Publicado en línea: 12 jun 2017

Páginas: 163 - 171

Recibido: 03 ene 2017

Aceptado: 26 may 2017

DOI: https://doi.org/10.1515/jvetres-2017-0021

Palabras clave
ApxIV, ELISA, oral fluid, antibody

© 2017 Wendy González et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detection of Actinobacillus pleuropneumoniae ApxIV toxin antibody in serum and oral fluid specimens from pigs inoculated under experimental conditions

Publicado en línea: 12 jun 2017

Páginas: 163 - 171

Recibido: 03 ene 2017

Aceptado: 26 may 2017

DOI: https://doi.org/10.1515/jvetres-2017-0021

Palabras claveApxIV, ELISA, oral fluid, antibody

© 2017 Wendy González et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
ApxIV, ELISA, oral fluid, antibody