<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The study objective was to develop and evaluate a new TaqMan multiplex real-time PCR method for <italic>Salmonella</italic>, <italic>L. monocytogenes</italic>, and verotoxigenic <italic>Escherichia coli</italic> (VTEC) detection in slaughtered animal carcasses.<bold>Material and Methods:</bold> The procedure included an enrichment step, DNA extraction, and two multiplex real-time PCRs. The first PCR detected the <italic>invA</italic> and <italic>hly</italic> genes of <italic>Salmonella</italic> and <italic>L. monocytogenes</italic> respectively, the second the <italic>vtx1</italic>, <italic>vtx2</italic>, and <italic>eae</italic> genes of VTEC.<bold>Results:</bold> The validation of this method resulted in 100% relative sensitivity, specificity, and accuracy as compared to the reference ISO methods. The limit of detection per swab sample was established at 1 cfu for <italic>Salmonella</italic> and <italic>L. monocytogenes</italic> and 2 cfu for VTEC. The authors analysed 265 slaughterhouse-collected swabs from cattle, pig, and poultry carcasses. Among 125 from cattle, 51 were positive for VTEC, 29 for <italic>Salmonella</italic>, and 1 for <italic>L. monocytogenes</italic>. Among swabs from pig carcasses (n = 95), three, two, and one sample were positive for these pathogens respectively. None of the microorganisms tested for was identified in 45 samples of poultry origin.<bold>Conclusion:</bold> The obtained results showed that the method developed can rapidly identify the main bacterial pathogens that may contaminate carcasses of food-producing animals.</abstract>

Introduction: The study objective was to develop and evaluate a new TaqMan multiplex real-time PCR method for Salmonella, L. monocytogenes, and verotoxigenic Escherichia coli (VTEC) detection in slaughtered animal carcasses.Material and Methods: The procedure included an enrichment step, DNA extraction, and two multiplex real-time PCRs. The first PCR detected the invA and hly genes of Salmonella and L. monocytogenes respectively, the second the vtx1, vtx2, and eae genes of VTEC.Results: The validation of this method resulted in 100% relative sensitivity, specificity, and accuracy as compared to the reference ISO methods. The limit of detection per swab sample was established at 1 cfu for Salmonella and L. monocytogenes and 2 cfu for VTEC. The authors analysed 265 slaughterhouse-collected swabs from cattle, pig, and poultry carcasses. Among 125 from cattle, 51 were positive for VTEC, 29 for Salmonella, and 1 for L. monocytogenes. Among swabs from pig carcasses (n = 95), three, two, and one sample were positive for these pathogens respectively. None of the microorganisms tested for was identified in 45 samples of poultry origin.Conclusion: The obtained results showed that the method developed can rapidly identify the main bacterial pathogens that may contaminate carcasses of food-producing animals.

Multiplex real-time PCRs for detection of <italic>Salmonella</italic>, <italic>Listeria monocytogenes</italic>, and verotoxigenic <italic>Escherichia coli</italic> in carcasses of slaughtered animals

Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy,

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Multiplex real-time PCRs for detection of , , and verotoxigenic  in carcasses of slaughtered animals

Introduction: The study objective was to develop and evaluate a new TaqMan multiplex real-time PCR method for Salmonella, L. monocytogenes, and verotoxigenic...

Multiplex real-time PCRs for detection of Salmonella, Listeria monocytogenes, and verotoxigenic Escherichia coli in carcasses of slaughtered animals

Publicado en línea: 23 sept 2016

Páginas: 287 - 292

Recibido: 09 may 2016

Aceptado: 29 ago 2016

DOI: https://doi.org/10.1515/jvetres-2016-0044

Palabras clave
carcasses, foodborne pathogens, multiplex real-time PCRs, identification, food safety

© 2016 Edyta Denis et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Multiplex real-time PCRs for detection of Salmonella, Listeria monocytogenes, and verotoxigenic Escherichia coli in carcasses of slaughtered animals

Publicado en línea: 23 sept 2016

Páginas: 287 - 292

Recibido: 09 may 2016

Aceptado: 29 ago 2016

DOI: https://doi.org/10.1515/jvetres-2016-0044

Palabras clavecarcasses, foodborne pathogens, multiplex real-time PCRs, identification, food safety

© 2016 Edyta Denis et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
carcasses, foodborne pathogens, multiplex real-time PCRs, identification, food safety