<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Introduction: The differentially expressed proteins between healthy cows and those with footrot were identified to explore changes in protein profiles associated with the disease. Material and Methods: Out of 36 cows selected for the experiment, 18 footrot-affected cows were included in the treatment group (group T) and 18 unaffected cows were included in the control group (group C). Plasma samples from groups T and C were subjected to two-dimensional electrophoresis analysis and differentially expressed proteins were identified by matrix-assisted laser desorption/ionisation tandem time-of-flight mass spectrometry. Bioinformatics, including gene ontology analysis and pathway analysis, was used for analysing all proteins. Results: Out of 63 spots identified by 2DE, 33 were selected for mass spectrum analysis, which identified 11 differentially expressed proteins in 26 spots. Footrot led to changes in profiles in plasma proteins that were classified to the pathway of inflammatory response, complement, and blood coagulation, among others. Conclusion: This study provides evidence of the defence mechanisms of cows with footrot to explore strategies for treatment.</p></abstract>

Introduction: The differentially expressed proteins between healthy cows and those with footrot were identified to explore changes in protein profiles associated with the disease. Material and Methods: Out of 36 cows selected for the experiment, 18 footrot-affected cows were included in the treatment group (group T) and 18 unaffected cows were included in the control group (group C). Plasma samples from groups T and C were subjected to two-dimensional electrophoresis analysis and differentially expressed proteins were identified by matrix-assisted laser desorption/ionisation tandem time-of-flight mass spectrometry. Bioinformatics, including gene ontology analysis and pathway analysis, was used for analysing all proteins. Results: Out of 63 spots identified by 2DE, 33 were selected for mass spectrum analysis, which identified 11 differentially expressed proteins in 26 spots. Footrot led to changes in profiles in plasma proteins that were classified to the pathway of inflammatory response, complement, and blood coagulation, among others. Conclusion: This study provides evidence of the defence mechanisms of cows with footrot to explore strategies for treatment.

2-DE-MS based proteomic investigation of dairy cows with footrot

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University Daqing High-tech Industrial Development Zone, Daqing 163319, People’s Republic of China

Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030, People’s Republic of China

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"2-DE-MS based proteomic investigation of dairy cows with footrot"}

Introduction: The differentially expressed proteins between healthy cows and those with footrot were identified to explore changes in protein profiles...

2-DE-MS based proteomic investigation of dairy cows with footrot

Publicado en línea: 29 abr 2016

Páginas: 63 - 69

Recibido: 28 sept 2015

Aceptado: 19 feb 2016

DOI: https://doi.org/10.1515/jvetres-2016-0010

Palabras clave
dairy cows, 2-DE-MS, proteomic, footrot

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

2-DE-MS based proteomic investigation of dairy cows with footrot

Publicado en línea: 29 abr 2016

Páginas: 63 - 69

Recibido: 28 sept 2015

Aceptado: 19 feb 2016

DOI: https://doi.org/10.1515/jvetres-2016-0010

Palabras clavedairy cows, 2-DE-MS, proteomic, footrot

© 2016

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
dairy cows, 2-DE-MS, proteomic, footrot