<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Disinfection of plant material is the most important step of the tissue culture protocol. In this process, an attempt is made to eliminate microbial contaminants from the surface and interior of plant material, thus giving the explant a fighting chance at survival in vitro. Initial cultures of Anthurium species and cultivars, which are usually established from ex vitro material grown in a greenhouse, pots or in the field, easily contaminate the in vitro milieu. This review highlights the differences in disinfection protocols that exist for different species or cultivars of Anthurium. The protocol needs to be adjusted based on the material used: spadices, spathes, seeds, leaves, or roots. Regrettably, most of the currently published protocols, derived from a literature that spans over 100 published papers, have numerous weaknesses and flaws in the information provided pertaining to disinfection and infection levels. Advice for future Anthurium researchers should thus be followed cautiously.</p></abstract>

Disinfection of plant material is the most important step of the tissue culture protocol. In this process, an attempt is made to eliminate microbial contaminants from the surface and interior of plant material, thus giving the explant a fighting chance at survival in vitro. Initial cultures of Anthurium species and cultivars, which are usually established from ex vitro material grown in a greenhouse, pots or in the field, easily contaminate the in vitro milieu. This review highlights the differences in disinfection protocols that exist for different species or cultivars of Anthurium. The protocol needs to be adjusted based on the material used: spadices, spathes, seeds, leaves, or roots. Regrettably, most of the currently published protocols, derived from a literature that spans over 100 published papers, have numerous weaknesses and flaws in the information provided pertaining to disinfection and infection levels. Advice for future Anthurium researchers should thus be followed cautiously.

Disinfection procedures for <italic>in vitro</italic> propagation of <italic>Anthurium</italic>

P.O. Box 7, Miki-Cho Post Office, Ikenobe 3011-2, Kagawa-Ken, 761-0799,

Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement South China Botanical Garden

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Disinfection procedures for  propagation of 

Disinfection of plant material is the most important step of the tissue culture protocol. In this process, an attempt is made to eliminate microbial...

Disinfection procedures for in vitro propagation of Anthurium

Publicado en línea: 30 abr 2015

Páginas: 3 - 14

Recibido: 03 mar 2015

Aceptado: 04 abr 2015

DOI: https://doi.org/10.1515/fhort-2015-0009

Palabras clave
contamination, seed, sodium hypochlorite, spadix, spathe, tissue culture

© by Jaime A. Teixeira da Silva

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Disinfection procedures for in vitro propagation of Anthurium

Publicado en línea: 30 abr 2015

Páginas: 3 - 14

Recibido: 03 mar 2015

Aceptado: 04 abr 2015

DOI: https://doi.org/10.1515/fhort-2015-0009

Palabras clavecontamination, seed, sodium hypochlorite, spadix, spathe, tissue culture

© by Jaime A. Teixeira da Silva

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
contamination, seed, sodium hypochlorite, spadix, spathe, tissue culture