<abstract xmlns="http://www.w3.org/1999/xhtml"><p> A quite simple and rapid TLC-densitometric method for the identification of α-escin (Aescin) in bulk drug substances was developed. In so doing, different chromatographic conditions, including various mobile and stationary phases, were tested. A TLC densitometric determination of the examined compound was performed without using visualizing reagent, yet with the use of appropriate dipping reagents, in order to obtain reliable UV-densitometric measurements of α-escin - a substance which has weak chromophore groups. Herein, the application of a mobile phase containing n-butanolacetic acid-water in volume composition 30:7:13, the use of silica gel 60F<sub>254</sub> plates with concentrating zone, and subsequent application of 10% sulphuric acid in ethanol or 5% vanillin in methanol/sulphuric acid, respectively, provided the best results in a TLCdensitometric study of α-escin. The described method was successfully employed to identify α-escin in commercial samples that were in an oral dosage form (tablets) and also in the form of gel containing 20 mg of α-escin.</p></abstract>

A quite simple and rapid TLC-densitometric method for the identification of α-escin (Aescin) in bulk drug substances was developed. In so doing, different chromatographic conditions, including various mobile and stationary phases, were tested. A TLC densitometric determination of the examined compound was performed without using visualizing reagent, yet with the use of appropriate dipping reagents, in order to obtain reliable UV-densitometric measurements of α-escin - a substance which has weak chromophore groups. Herein, the application of a mobile phase containing n-butanolacetic acid-water in volume composition 30:7:13, the use of silica gel 60F254 plates with concentrating zone, and subsequent application of 10% sulphuric acid in ethanol or 5% vanillin in methanol/sulphuric acid, respectively, provided the best results in a TLCdensitometric study of α-escin. The described method was successfully employed to identify α-escin in commercial samples that were in an oral dosage form (tablets) and also in the form of gel containing 20 mg of α-escin.

TLC-densitometric analysis of α-escin in bulk drug substance and in pharmaceutical dosage forms

Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Silesia in Katowice, 4 Jagiellonska, 41-200 Sosnowiec, Poland

R&D Analytical Chemistry Department, Pharmaceutical Research Institute, 8 Rydygiera, 01-793 Warsaw, Poland

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This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

A quite simple and rapid TLC-densitometric method for the identification of α-escin (Aescin) in bulk drug substances was developed. In so doing, different...

TLC-densitometric analysis of α-escin in bulk drug substance and in pharmaceutical dosage forms

Publicado en línea: 26 nov 2015

Páginas: 186 - 191

Recibido: 08 ene 2015

Aceptado: 16 sept 2015

DOI: https://doi.org/10.1515/cipms-2015-0069

Palabras clave
α-escin, TLC, densitometry, triterpene compounds

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

TLC-densitometric analysis of α-escin in bulk drug substance and in pharmaceutical dosage forms

Publicado en línea: 26 nov 2015

Páginas: 186 - 191

Recibido: 08 ene 2015

Aceptado: 16 sept 2015

DOI: https://doi.org/10.1515/cipms-2015-0069

Palabras claveα-escin, TLC, densitometry, triterpene compounds

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
α-escin, TLC, densitometry, triterpene compounds