<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Objective</bold>. Amflora potato, event EH92-527-1 was genetically modified to produce only the amylopectin component from starch composition. The presence of the transgenic potato in the food on Romanian market should be verified although the Amflora potato uses for industrial purposes and animal feed. The aim of this study was to detect the presence of the Amflora potato in the food or feed products.<bold>Methods</bold>. For this purpose, five samples of potato tubers and four samples of chips were analyzed. DNA isolation was performed with E.Z.N.A. ® MicroElute Genomic DNA kit (Omega Bio-Tek, USA). For identification a potential presence of the recombinant DNA in the food samples it was used GMOIdent RT Event EH92-527-1 Potato kit (Eurofins GeneScan, Germany), that is a real-time PCR kit for qualitative event-specific detection of AmfloraTM EH92-527-1 potato.<bold>Results</bold>. Amflora transgenic potato was not detected in any of analyzed samples.<bold>Conclusions</bold>. The results of real-time PCR method confirm the absence of Amflora event EH92-527-1 in all potato tubers and chips analyzed samples.</abstract>

Objective. Amflora potato, event EH92-527-1 was genetically modified to produce only the amylopectin component from starch composition. The presence of the transgenic potato in the food on Romanian market should be verified although the Amflora potato uses for industrial purposes and animal feed. The aim of this study was to detect the presence of the Amflora potato in the food or feed products.Methods. For this purpose, five samples of potato tubers and four samples of chips were analyzed. DNA isolation was performed with E.Z.N.A. ® MicroElute Genomic DNA kit (Omega Bio-Tek, USA). For identification a potential presence of the recombinant DNA in the food samples it was used GMOIdent RT Event EH92-527-1 Potato kit (Eurofins GeneScan, Germany), that is a real-time PCR kit for qualitative event-specific detection of AmfloraTM EH92-527-1 potato.Results. Amflora transgenic potato was not detected in any of analyzed samples.Conclusions. The results of real-time PCR method confirm the absence of Amflora event EH92-527-1 in all potato tubers and chips analyzed samples.

Screening the Presence of Amflora Transgenic Potato in Food or Feed Products

Cell Biology and Microbiology Department, University of Medicine and Pharmacy,

Acta Marisiensis - Seria Medica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Screening the Presence of Amflora Transgenic Potato in Food or Feed Products"}

Objective. Amflora potato, event EH92-527-1 was genetically modified to produce only the amylopectin component from starch composition. The presence of the...

Screening the Presence of Amflora Transgenic Potato in Food or Feed Products

Publicado en línea: 30 dic 2016

Páginas: 392 - 394

Recibido: 21 oct 2016

Aceptado: 28 nov 2016

DOI: https://doi.org/10.1515/amma-2016-0051

Palabras clave
Amflora, screening, real-time PCR, food products

© 2016 Curticăpean Manuela-Claudia, published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Screening the Presence of Amflora Transgenic Potato in Food or Feed Products

Publicado en línea: 30 dic 2016

Páginas: 392 - 394

Recibido: 21 oct 2016

Aceptado: 28 nov 2016

DOI: https://doi.org/10.1515/amma-2016-0051

Palabras claveAmflora, screening, real-time PCR, food products

© 2016 Curticăpean Manuela-Claudia, published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
Amflora, screening, real-time PCR, food products