<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Objective</bold>: In this study we report the development of a simple, rapid and efficient capillary electrophoresis method for the simultaneous determination of atorvastatin, fluvastatin, lovastatin and simvastin.<bold>Methods</bold>: Capillary zone electrophoresis proved to be efficient for the simultaneous separation of atorvastatin and fluvastatin, but could not resolve the determination of lovastatin and simvastatin. The simultaneous separation of all four statins was achieved by applying a micellar electrokinetic chromatographic method, after transforming lovastatin and simvastatin in β-hydroxyl acid forms through alkaline hydrolysis. The optimum electrophoretic conditions and analytical parameters were investigated and the analytical performances of the method were verified with regard to linearity, precision, accuracy, LOD and LOQ.<bold>Results</bold>: The optimum electrophoretic separation conditions were: 25 mM sodium tetraborate with 25 mM sodium dodecyl sulphate buffer electrolyte at pH 9.5, applied voltage + 25 kV, separation temperature 25 °C, injection pressure/time 50 mbar/1 minutes, UV detection at 230 nm. Using the optimized electrophoretic conditions we succeeded in the simultaneous determination of the four statins in approximately 3 minutes, the order of migration being: atorvastatin, fluvastatin, lovastatin, simvastatin. The proposed method has been applied to the determination of the analytes in pharmaceutical tablets formulations.<bold>Conclusions</bold>: The capillary electrophoretic method developed in the present work proved to be suitable for the routine analysis of statins and can be adopted as quality control protocol in pharmaceutical analysis.</abstract>

Objective: In this study we report the development of a simple, rapid and efficient capillary electrophoresis method for the simultaneous determination of atorvastatin, fluvastatin, lovastatin and simvastin.Methods: Capillary zone electrophoresis proved to be efficient for the simultaneous separation of atorvastatin and fluvastatin, but could not resolve the determination of lovastatin and simvastatin. The simultaneous separation of all four statins was achieved by applying a micellar electrokinetic chromatographic method, after transforming lovastatin and simvastatin in β-hydroxyl acid forms through alkaline hydrolysis. The optimum electrophoretic conditions and analytical parameters were investigated and the analytical performances of the method were verified with regard to linearity, precision, accuracy, LOD and LOQ.Results: The optimum electrophoretic separation conditions were: 25 mM sodium tetraborate with 25 mM sodium dodecyl sulphate buffer electrolyte at pH 9.5, applied voltage + 25 kV, separation temperature 25 °C, injection pressure/time 50 mbar/1 minutes, UV detection at 230 nm. Using the optimized electrophoretic conditions we succeeded in the simultaneous determination of the four statins in approximately 3 minutes, the order of migration being: atorvastatin, fluvastatin, lovastatin, simvastatin. The proposed method has been applied to the determination of the analytes in pharmaceutical tablets formulations.Conclusions: The capillary electrophoretic method developed in the present work proved to be suitable for the routine analysis of statins and can be adopted as quality control protocol in pharmaceutical analysis.

Determination of HMG-CoA reductase inhibitors by micellar electrokinetic chromatography

University of Medicine and Pharmacy Tirgu Mures, Romania

Acta Marisiensis - Seria Medica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Objective: In this study we report the development of a simple, rapid and efficient capillary electrophoresis method for the simultaneous determination of...

Determination of HMG-CoA reductase inhibitors by micellar electrokinetic chromatography

Publicado en línea: 20 may 2016

Páginas: 187 - 191

Recibido: 11 nov 2015

Aceptado: 19 dic 2015

DOI: https://doi.org/10.1515/amma-2016-0006

Palabras clave
capillary electrophoresis, statins, micellar electrokinetic capillary chromatography, simultaneous determination, pharmaceutical analysis

© 2016 Mircia Eleonora et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Determination of HMG-CoA reductase inhibitors by micellar electrokinetic chromatography

Publicado en línea: 20 may 2016

Páginas: 187 - 191

Recibido: 11 nov 2015

Aceptado: 19 dic 2015

DOI: https://doi.org/10.1515/amma-2016-0006

Palabras clavecapillary electrophoresis, statins, micellar electrokinetic capillary chromatography, simultaneous determination, pharmaceutical analysis

© 2016 Mircia Eleonora et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
capillary electrophoresis, statins, micellar electrokinetic capillary chromatography, simultaneous determination, pharmaceutical analysis